Glycolysis

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Author:
ccraigr
ID:
204465
Filename:
Glycolysis
Updated:
2013-03-02 14:53:22
Tags:
glycolysis biochem metabolic pathway
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Description:
stepwise description of clycolysis
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  1. Describe step 1
    Include reactants, products, and required enzymes.
    • Cyclic glucose is phosphorylated by ATP at C6
    • Reactants: Glucose+ATP
    • Products: Glucose 6 Phosphate & ADP
    • Enzyme: Hexokinase
    • This is a high energy step.
  2. Describe step 2. Include reactants, products, and required enzymes.
    • Glucose 6 phosphate is opened and then closed at C2 to form fructose (6membered ring to 5 membered ring)
    • Reactants: G6P
    • Products: F6P
    • Enzyme: Phophohexose Isomerase
  3. Describe step 3. Include reactants, products, and required enzymes.
    • Fructose 6-Phosphate is phosphrylated by ATP at C1 to make Fructose 1,6-Bisphophate.
    • Reactants: F6P, ATP
    • Products: F16BP, ADP
    • Enzyme: Phosphofructokinase 1
    • This is a high energy step
  4. Describe step 4. Include reactants, products, and required enzymes.
    • Fructose 1,6-Bisphosphate is opened and attacked by Lys-NH2 (Shiff base from C=O to C=N). This kicks off 1 GAP and then reforms the C=O for DHAP.
    • Reactants: F16BP, Lys-NH2
    • Products: DHAP, GAP, Lys-NH2
    • Enzyme: Aldolase
  5. Describe step 5. Include reactants, products, and required enzymes.
    • DHAP is turned into 2nd GAP (Double bond on C2 swaps places with H on C3-OH)
    • Reactants: DHAP
    • Products: GAP
    • Enzyme: Triose Phosphate Isomerase
  6. Describe step 6. Include reactants, products, and required enzymes.
    • GAP C1 is changed from H-CR=O into PO-CR=O. NAD+¬†SCys and H2O help the transition. Now there is a P on both ends.
    • Reactants: GAP, SCys, NAD+, H2O
    • Products: 1,3-Bisphosphoglycerate, SCys, NADH
    • Enzyme: GAP Dehydrogenase
  7. Describe step 7. Include reactants, products, and required enzymes.
    • Phosphate on C1 is pulled off by ADP, forming ATP.
    • Reactants: 1,3-Bisphosphoglycerate, ADP
    • Products: 3-Phosphoglycerate, ATP
    • Enzyme: Phosphoglycerate kinase
  8. Describe step 8. Include reactants, products, and required enzymes.
    • The C3 phosphate is moved to C2 by two Histine pyridines.
    • Reactants: 3-Phosphoglycerate, 2 Histine transfer pyridines, 1 with a Phosphate group.
    • Products: 2-Phosphoglycerate, 2 Histine transfer pyridines, 1 with a Phosphate group.
    • Enzyme: Phosphoglycerate Mutase
  9. Describe step 9. Include reactants, products, and required enzymes.
    • An H is pulled off of C2, and a double bond forms between C2 and C3. Magnesium stabalizes this step.
    • Reactants: 2-Phosphoglycerate, 2Mg2+ (for stability)
    • Products: Phosphoenolpyruvate, 2Mg 2+
    • Enzyme: Enolase
  10. Describe step 10. Include reactants, products, and required enzymes.
    • ADP pulls of C2 Phosphate. Non Enzyme catalyzed tautomerization moves C2=C3 into C2=O (begins as: C2-OH. The H moves to C3)
    • Reactants: Phosphoenol pyruvate, ADP
    • Products: Pyruvate, ATP
    • Enzyme: Pyruvate Kinase
    • This is a high energy step.

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