Biotechnology and genetic manipulation

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Author:
athederan
ID:
20530
Filename:
Biotechnology and genetic manipulation
Updated:
2010-05-24 05:48:59
Tags:
biotech
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Description:
final review
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  1. DNA technology
    • Using natural processes of replication, transcription, translation, etc. to manipulate genes in living cells.
    • Need to look at amplification, detection, splicing, and vectors in cells
  2. tools of the biotechnologist
    • Enzymes that interact with DNA and RNA
    • Plasmid DNA
    • Host organisms
    • Electrophoresis
  3. Recombinant DNA
    • most cells- DNA is in the nucleus and forms part of the genetic make up
    • some cells may have other small pieces of DNA (plasmids)
    • These circular pieces of DNA can be extracted from the cells, changed and replicated giving new properties to the cell: Recombinant DNA
  4. Plasmid DNA
    • self - replication pieces of DNA that are not tied to the replication cycles of the nuclear DNA
    • may be many copies of a plasmid inside a cell
    • occur naturally in cells
    • may be responsible for unique properties like drug resistance, toxin production or other non essiental traits
    • need to have: orgin of replication, promotor (host cell), Marker (antibiotic resistance)
  5. Endonucleases
    • enzymes that can cut DNA in the middle of the strand
    • leaves ragged ends that could be specifically bound to other pieces of DNA
    • allows ability to cut and paste different pieces of DNA together
    • this is how insulin and penicillin and many antibiotics are produced

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