LAB EXAM 2

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sandovalfj
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206211
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LAB EXAM 2
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2013-03-19 22:03:41
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LAB
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    • NAME- STARCH HYDROLYSIS
    • SUBSTRATE - STARCH
    • ENZYME - AMYLASE
    • POSITIVE REACTION - BLUE/BLACK AND CLEARING ZONE
    • NEGATIVE REACTION - AMBER
    • REAGANT - IODINE
    • PRODUCT - MONO OR DISACCHARIDES
    • EXOENZYME AND EXTRACELLULAR DIGESTION
    • NAME CASEIN HYDROLYSIS
    • SUBSTRATE - CASEIN
    • ENZYME - CASEINASE
    • POSITIVE REACTION - CLEARING ZONES = HYDROLYSIS
    • NEGATIVE REACTION - NO CLEARING
    • PRODUCT - DIPEPTIDES AND AMINO ACIDS
    • EXOENZYME AND EXTRACELLULAR DIGESTION
    • NAME - PHENOL RED BROTH
    • SUBSTRATE -CARBOHYDRATE
    • PRODUCT - ACID
    • POSITIVE - YELLOW (ACIDIC) = SUGAR FERMENTATION
    • NEGATIVE - RED (BASIC)
    • GAS PRODUCTION = BIG BUBBLE IN DURHEIM TUBE
    • ENDOENZYME
    • NAME - TRIPLE SUGAR IRON
    • A/A - ACID/ACID - YELLOW/YELLOW (SUGAR DIGESTION)
    • A/K - ACID/ALKALINE - YELLOW/RED (REVERSION)
    • K/K - ALKALINE/ALKALINE - RED/RED (PROTEIN DIGESTION)
    • H2S REACTION - BLACK PRECIPITATE  H2S + Fe -> FeS
    • CO2 +  CRACKS IN AGAR
    • NAME - V.P.
    • REAGENT - BARRITTS A & B
    • POSITIVE - RED
    • NEGATIVE - YELLOW
    • PRODUCTS - ACETOIN AND BUTANEDOIL
    • -V.P. -> + M.R.
    • - M.R. -> + V.P.
    • NAME - M.R.
    • POSITIVE - RED (ACIDIC)
    • NEGATIVE - YELLOW (BASIC)
    • PRODUCTS - MIXED ACIDS
    • - V.P. -> + M.R.
    • - M.R. -> + V.P.
    • NAME - GELATIN
    • SUBSTRATE - GELATIN
    • ENZYME - GELATINASE
    • PRODUCTS - AMINO ACIDS/ DIPEPTIDES
    • POSITIVE - LIQUIFIED
    • Staphylococcus aureus
    • NEGATIVE - SOLID
    • staphylococcus epidermis
    • CHILL TO AVOID FALSE POSITIVE
    • EXOENZYME AND EXTRACELLULAR DIGESTION
    • NAME - SIM / INDOLE
    • SUBSTRATE - TRYTOPHAN
    • ENZYME - TRYTOPHANASE
    • PRODUCT - INDOLE POSITIVE (PINK/RED)
    • REAGENT - KOVACS
    • POSITIVE MOTILITY - CLOUDINESS AWAY FORM STAB
    • H2S PRODUCTION - H2S + Fe -> FeS = BLACK PRECIPITATE CAN'T BE DETERMINED IF WHOLE TUBE IS BLACK
    • NAME - BLOOD AGAR
    • BETA HEMOLYSIS - CLEARING AROUND COLONY
    • GROUP A Streptococcus pyongenes SENSITIVE TO BACITRACIN
    • ALPHA HEMOLYSIS - YELLOW/BROWN COLOR
    • GAMMA HEMOLYSIS - GROWTH NO CLEARING
    • ENRICHED WITH BLOOD
    • DIFFERENTIAL - ALLOWS FOR DIFFERENT HEMOLYTIC REACTION
    • EXOENZYME AND EXTRACELLULAR DIGESTION
    • NAME - MANNITOL SALTS
    • SUBSTRATE - MANNITOL
    • SELECTIVE FOR Staphylococcus GENERA DUE TO SALT INTOLERANCE
    • DIFFERENTIAL - MANNITOL FERMENTATION
    • PRODUCT - ACID
    • POSITIVE - YELLOW (ACIDIC)
    • YELLOW ACID FERMENTATION - Staphylococcus aureus
    • GROWTH BUT NO YELLOW - Staphylococcus
    • ENDOENZYME
    • NAME - EOSIN METHYLENE BLUE
    • SELECTIVE FOR GRAM NEGATIVE
    • DIFFERENTIAL -
    • NON LACTOSE FERMENTERS - WHITE COLONIES
    • LACTOSE FERMENTERS - PINK/PURPLE
    • STRONG LACTOSE FERMENTERS - METALLIC GREEN Escherichia coli
    • ENDOENZYME
    • NAME - MACCONKEY
    • SUBSTRATE - LACTOSE
    • ENZYME - LACTASE
    • SELECTIVE - GRAM NEGATIVE B/C OF BILE SALTS
    • DIFFERENTIAL -
    • WHITE - NON FERMENTATION OF LACTOSE
    • PINK - FERMENTATION OF LACTOSE
    • ENDOENZYME
    • NAME - CATALASE
    • SUBSTRATE - HYDROGEN PEROXIDE
    • ENZYME - CATALASE
    • PRODUCT - H20 + O2
    • POSITIVE - BUBBLES / FIZZING
    • NEGATIVE - NO FIZZING
    • EXOENZYME AND EXTRACELLULAR DIGESTION
    • NAME - LIPID HYDROLYSIS / SPIRIT BLUE
    • SUBSTRATE - LIPIDS
    • ENZYME - LIPASE
    • PRODUCTS - GLYCEROL AND FATTY ACIDS
    • POSITIVE - CLEARING ZONE = LIPID HYDROLYSIS
    • NEGATIVE - NO CLEARING
    • EXOENZYME AND EXTRACELLULAR DIGESTION
    • NAME - DNase
    • SUBSTRATE - DNA
    • ENZYME - DNase
    • PRODUCTS - NUCLEOTIDES
    • POSITIVE - CLEARING ZONES BREAKDOWN OF DNA
    • Staphylococcus aureus
    • CLINICALLY IMPORTANT - BREAKDOWN OUR DNA
    • EXOENZYME AND EXTRACELLULAR DIGESTION
    • NAME - CITRATE
    • POSITIVE - BLUE = BASIC
    • NEGATIVE - GREEN = NEUTRAL
    • CARBON SOURCE
    • CITRATE -> CITRASE -> PYRUVATE -> BASIC PRODUCTS
    • ENDOENZYME
    • NAME - UREA
    • SUBSTRATE - UREA
    • ENZYME - UREASE
    • PRODUCT - AMMONIA
    • POSITIVE - PINK
    • NEGATIVE - YELLOW
    • Proteus vulgaris
    • ENDOENZYME
    • NAME - NITRATE
    • CAUSES EXTENSIVE TISSUE INFECTION B/C IN ANAEROBIC USE NITROGEN AS FINAL ELECTRON ACCEPTOR
    • NO3(NITRATE) -> NITRATE REDUCTASE -> NO2(NITRITE) -> NITRITE REDUCTASE -> N20 OR N2
    • REAGENTS ADDED TURNS RED = NITRITE PRESENT USED NITRATE REDUCTASE
    • REAGENTS ADDED + ZINC TURNS RED = NO REACTION, NOTHING PRESENT  CHEMICAL REACTION ONLY
    • REAGENTS ADDED + ZINC  REMAINS CLEAR = NITRATE & NITRITE REDUCTIONS OCCURRED N20 OR N2 PRESENT
    • ENDOENZYME
    • NAME = RAPID STREP TEST
    • POSITIVE - BLUE AND PINK BANDS
    • NEGATIVE - PINK BAND
  1. COLIFORM
    • GRAM NEGATIVE & LACTOSE FERMENTER
    • COME FROM RAW SEWAGE
  2. ENTRIC
    • GRAM NEGATIVE & LACTOSE FERMENTERS
    • FOUND IN INTESTINES
  3. THE ABILITY TO NEUTRALIZE OXYGEN
    • CAPABILITY SHARED BY MANY AEROBIC AND FACULTATIVE ANAEROBIC ORGANISMS
    • LACKING IN STRICT ANAEROBES
  4. EXTRACELLULAR DIGESTION
    DIGESTION OUTSIDE THE CELL
  5. GLYCOSIDIC BOND
    A TYPE OF COVALENT BOND THAT JOINS A CARBOHYDRATE (SUGAR) MOLECULE TO ANOTHER GROUP, WHICH MAY OR MAY NOT BE ANOTHER CARBOHYDRATE
  6. ANTIGEN
    FOREIGN COMPONENT
  7. ANTIBODY
    THE BODY'S RESPONSE TO FOREIGN COMPOUND
  8. SEROLOGY
    REACTION OF ANTIGEN AND ANTIBODY

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