chapter 3 micro

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Anonymous
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209338
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chapter 3 micro
Updated:
2013-03-25 02:12:51
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  1. negative, indirect or back ground staining
    mixing bacteria with an acidic acid or dye called chromogen such as nigrosin, india ink, or eosin then spreading out the mixture on a slide to form a film
  2. Negative staining procedure
    • 1. add two drops of culture
    • 2. add 2 drops of nigrosin 
    • 3. slide second slide across to spread out
    • 4. let air dry
  3. smear preperation
    • 1. drop of water and bacteria 
    • 2. spread out mixture
    • 3. air dry 
    • 4. heat fix
  4. bacterial smear
    a dried preperation of bacterial cells on a glass slide
  5. simple staining preperation
    1. add stain (crystal violet 20 sec, carbolfuchsin 5-10 sec, methylen blue 1 min)

    • 2. water off
    • 3. blot off
  6. Morphology
    one individual cell shape
  7. arrangement
    groups ways you find multiple together.
  8. Complex stain
    several aggents
  9. differential stain
    allows for two or more outcomes
  10. Gram positive
    thick walls retain primary Purple
  11. Gram Negative
    looses primary an becomes clear. stains red
  12. Gram staining
    divides into two groups. Gram positive and negative
  13. Primary stain
    first step crystal violet dye
  14. Mordant
    iodine solution
  15. counter stained
    sahranin will stain colorless gram negative bacteria pink  does not alter th positive
  16. Gram variable
    might have + and - do many tries
  17. Gram stain preperation
    • 1. prepare a heat fixed smear
    • 2. place slide on rack
    • 3. flood the smear with crystal violet for 30 sec
    • 4. rince with water for 5 seconds
    • 5. cover with iodine for one minute
    • 6. rinse
    • 7. docolorize for 15- 30 seconds
    • 8. rinse
    • 9. add safranin for about 60- 80 seconds 
    • 10 rinse 
    • 11. blot dry.

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