Fundamental Hematology Principles

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Anonymous
ID:
209852
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Fundamental Hematology Principles
Updated:
2013-03-27 12:52:26
Tags:
Blood Composition Homeostasis RBC Indices Parameters Platelets Stains
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Description:
Blood Composition, Homeostasis, RBC Indices, RBC Parameters, Platelets, Stains
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  1. Name the components of whole blood
    • Erythrocytes
    • Leukocytes
    • Platelets
    • Plasma
  2. What makes up the buffy coat?
    Leukocytes
  3. What is plasma composed of?
    • 90% water
    • Proteins
    • Enzymes
    • Hormones
    • Lipids
    • Salts
  4. What is the body's tendency to move toward physiological stabiliy?
    Homeostasis
  5. What is the body/cellular water concentration, and is composed of 0.85% sodium chloride?
    Osmotic concentration
  6. What is the pH reference ranges, for both venous and arterial?
    • Venous: 7.36-7.41
    • Arterial: 7.38-7.44
  7. What is normal body temperature?
    37 degrees Celcius
  8. What is the MCV range?
    80-100 fL
  9. What does the MCV indicate?
    • The average/mean volume of RBC's
    • (Size)
  10. What is the calculation for MCV?
  11. What clinical conditions are associated with an increased MCV?
    • Megaloblastic anemia
    • Hemolytic anemia with retics
    • Liver disease
    • Normal in newborns
  12. What clinical conditions are associated with a decreased MCV?
    • Iron deficiency anemia
    • Thalassemia
    • Sideroblastic anemia
    • Lead poisoning
  13. What is the reference range of MCH?
    26-34 pg
  14. What is MCH an indicator of?
    Average weight of hemoglobin in individual RBCs
  15. What is the calculation of MCH?
  16. What clinical condition is associated with an increased MCH?
    Macrocytic anemia
  17. What clinical conditions are associated with a decreased MCH?
    • Microcytic anemia
    • Hypochromic anemia
  18. What is the reference range of MCHC?
    32-37 g/dL
  19. What is MCHC an indicator of?
    Average concentration of hemoglobin in grams per deciliter
  20. What is the calculation of MCHC?
  21. What does a value in between 32-37 g/dL MCHC indicate?
    Normochromic RBCs
  22. What does a value <32 g/dL MCHC indicate?
    • Hypochromic RBCs
    • Iron deficiency
    • Thalassemia
  23. What does a value >37 g/dL MCHC indicate?
    • Possible error in RBC or Hgb measurement
    • Presence of spherocytes
  24. What is the reference range for RDW?
    11.5-14.5%
  25. What is associated with increased RDW?
    • Anisocytosis (variation in size)
    • Post-transfusion
    • Post-treatment (Iron, B12, Folic acid treatment)
    • Idiopathic siderblastic anemia
  26. What is the reference range for HCT?
    • Males: 41-53%
    • Females: 36-46%
  27. What is the calculation for HCT?
  28. What is the reference range of Hgb?
    • Males: 13.5-17.5 g/dL
    • Females: 12.0-16.0 g/dL
  29. What is the reference range of platelets?
    150-450 x 109/L
  30. What is the reference range of MPV?
    6.8-10.2 fL
  31. What is the term for the amount of a cell type in realtion to other blood components?
    Relative count
  32. What is an increase in the percentage of lymphs and frequently associated with neutropenia?
    Relative lymphocytosis
  33. Describe relative polycythemia?
    RBCs appear increased due to decreased plasma volume
  34. What is the actual number of each cell type without respect to other blood components?
    Absolute count
  35. What is the most commonly used stain for peripheral blood smear?
    Wright's Stain
  36. What stains are included in Wright's stain?
    • Methylene blue
    • Eosin
    • Methanol fixative
  37. Name some polychrome stains
    • Wright
    • Giemsa
    • Leishman
    • Jenner
    • May-Grunwald
  38. What is the term for a type of stain used for nonvital (dead) cells?
    • Polychrome stain
    • (Romanowsky)
  39. Name a nonvital monchrome stain
    Prussian blue
  40. What is a stain that stains a specific cellular component?
    Nonvital monochrome stain
  41. What stain is used to stain iron granules in RBCs, histiocytes, and urine epithelial cells?
    Prussian blue
  42. What is the stain Prussian blue used for?
    To stain iron granules
  43. What type of stain is used on living cells for a specific cellular component?
    Supravital monochrome stain
  44. Name some supravital monochrome stains
    • New methylene blue
    • Neutral red with brillian cresyl green
  45. What is New methylene blue used to stain?
    • Used to see precipitate RNA in reticulocytes
    • Used to measure bone marrow erythropoiesis
  46. What is Neutral red with brillian cresyl green used to stain?
    Heinz bodies
  47. What clinical conditions are associated with Heinze Bodies?
    • G6PD deficiency
    • Unstable hemoglobin disorders

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