Staph and Strep
Card Set Information
Staph and Strep
Seabolt micro test
staph strep immunology
What does coagulase convert?
Fibrinogen to fibrin
What organism is Coagulase positive?
What do you verify the Staph tests with?
Mannitol and DNAse
What are the Mannitol and DNAse results for S. aureus?
What is S. aureus susceptibility to Novobiocin?
greater than or equal to 17mm
If the Coagulase test is negative what is the next test?
What Staph is novobiocin resistant?
What Staph is Novobiocin susceptible?
What is the Mannitol and DNAse results for S. epidermidis?
What are the Mannitol and DNAse test results for S. saprophyticus?
Mannitol+/- DNAse -
What organisms have beta hemolysis?
S. pyogenes, S. agalactiae
What organisms are alpha hemolysis?
S. pneumoniae, S. oralis
What organisms are non hemolytic?
S. bovis, and E. faecalis
What drug is used to during beta hemolysis?
Bacitracin (A disc)
What drug is used during Alpha hemolysis?
Optochin (P disc)
What drug is used for non hemolytic?
What organism is susceptible to Bacitracin?
If the strep is Bacitracin resistant what is the next test?
What produces a positive cAMP test?
What is optochin susceptible?
What is optochin resistant?
What is the next test if the Bile exculin test is positive?
NaCl or SF
What is NaCl or SF positive?
E. faecalis. Turbidity or Yellow
What is negative for NaCl or SF?
What does direct ELISA test for?
What does indirect ELISA test for?
What does immunodiffusion or ouchterlony assay allow?
Detects identity, partial identity or non identity of antigens/antibodies
What does immunofluorescence assay allow?
use a direct assay with only ONE reagent or indirect with TWO reagents
What is Immunoflouescence assay (FA) used for primarily?
What is the conformation of strep?
Gram positive cocci in chains
What kind of media should you plate strep on?
Blood and chocolate
What is Lancefield?
groups divided by serological testing of extractable carbohydrates
What are the Lancefield groups?
What groupd causes reumatic fever
What disease does Group B cause?
UTI in woman and diseases of newborns
What diseases does Group C cause?
Pathogens in animals. occasionally sinusitis in man
What disease does Group D cause?
Found in intestinal tract. Endocarditis UTI's and infections in wound
What are the virulence factors of Strep?
Hemolysins, Leucocidins, Erythrogenic toxin(A), Hyaluronidase, streptokinase, nucleases
What is the conformation of Staph?
Gram positive cocci in clusters
What is the indicator in DNAse?
What does S. aureus cause?
acne, boils, pneumonia, bone inflammation, endocarditis, food poisoning
What are the virulence factors of Staph?
Leukocidins, Hemolysins, Coagulase, Enterotoxin, DNAse and Lipases
How many times more likely are UTI's in females?
What are the normal mouth flora?
S. aureus, S. epidermidis, strep. mutans
What is the Snyder Test used for?
Determining the hosts susceptibility to dental carries
What is the indicator in the Snyder test?
What is immunology?
ability of an individual to resist infection by a particular microorganism due to natural or acquired defense mechanisms
What is a flourescence activated cell sorter
type of flow cytometry in which different cells within a suspension are detected based on flourescent antibody tag
used on not in living tissue
used on inanimate objects to inhibit growth
Chemicals that destroy bacteria in living tissue