Staph and Strep

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  1. Catalase positive
  2. Catalase Negative
  3. What does coagulase convert?
    Fibrinogen to fibrin
  4. What organism is Coagulase positive?
    S. aureus
  5. What do you verify the Staph tests with?
    Mannitol and DNAse
  6. What are the Mannitol and DNAse results for S. aureus?
    Both positive
  7. What is S. aureus susceptibility to Novobiocin?
    greater than or equal to 17mm
  8. If the Coagulase test is negative what is the next test?
  9. What Staph is novobiocin resistant?
    S. saprophyticus
  10. What Staph is Novobiocin susceptible?
    S. epidermidis
  11. What is the Mannitol and DNAse results for S. epidermidis?
    Both negative
  12. What are the Mannitol and DNAse test results for S. saprophyticus?
    Mannitol+/- DNAse -
  13. What organisms have beta hemolysis?
    S. pyogenes, S. agalactiae
  14. What organisms are alpha hemolysis?
    S. pneumoniae, S. oralis
  15. What organisms are non hemolytic?
    S. bovis, and E. faecalis
  16. What drug is used to during beta hemolysis?
    Bacitracin (A disc)
  17. What drug is used during Alpha hemolysis?
    Optochin (P disc)
  18. What drug is used for non hemolytic?
    Bile Esculin
  19. What organism is susceptible to Bacitracin?
    S. pyogenes
  20. If the strep is Bacitracin resistant what is the next test?
    cAMP test
  21. What produces a positive cAMP test?
    S, agalactiae
  22. What is optochin susceptible?
    S. pneumoniae
  23. What is optochin resistant?
    S. oralis?
  24. What is the next test if the Bile exculin test is positive?
    NaCl or SF
  25. What is NaCl or SF positive?
    E. faecalis. Turbidity or Yellow
  26. What is negative for NaCl or SF?
    S. bovis
  27. What does direct ELISA test for?
    Antigen detection
  28. What does indirect ELISA test for?
    antibody detection
  29. What does immunodiffusion or ouchterlony assay allow?
    Detects identity, partial identity or non identity of antigens/antibodies
  30. What does immunofluorescence assay allow?
    use a direct assay with only ONE reagent or indirect with TWO reagents
  31. What is Immunoflouescence assay (FA) used for primarily?
    Antigen detection
  32. What is the conformation of strep?
    Gram positive cocci in chains
  33. What kind of media should you plate strep on?
    Blood and chocolate
  34. What is Lancefield?
    groups divided by serological testing of extractable carbohydrates
  35. What are the Lancefield groups?
  36. What groupd causes reumatic fever
    Group A
  37. What disease does Group B cause?
    UTI in woman and diseases of newborns
  38. What diseases does Group C cause?
    Pathogens in animals. occasionally sinusitis in man
  39. What disease does Group D cause?
    Found in intestinal tract. Endocarditis UTI's and infections in wound
  40. What are the virulence factors of Strep?
    Hemolysins, Leucocidins, Erythrogenic toxin(A), Hyaluronidase, streptokinase, nucleases
  41. What is the conformation of Staph?
    Gram positive cocci in clusters
  42. What is the indicator in DNAse?
    Methyl green
  43. What does S. aureus cause?
    acne, boils, pneumonia, bone inflammation, endocarditis, food poisoning
  44. What are the virulence factors of Staph?
    Leukocidins, Hemolysins, Coagulase, Enterotoxin, DNAse and Lipases
  45. How many times more likely are UTI's in females?
  46. What are the normal mouth flora?
    S. aureus, S. epidermidis, strep. mutans
  47. What is the Snyder Test used for?
    Determining the hosts susceptibility to dental carries
  48. What is the indicator in the Snyder test?
    Cresol Green
  49. What is immunology?
    ability of an individual to resist infection by a particular microorganism due to natural or acquired defense mechanisms
  50. What is a flourescence activated cell sorter
    type of flow cytometry in which different cells within a suspension are detected based on flourescent antibody tag
  51. Antiseptics
    used on not in living tissue
  52. Disinfectants
    used on inanimate objects to inhibit growth
  53. Chemotherapeutic agents
    Chemicals that destroy bacteria in living tissue
Card Set:
Staph and Strep

staph strep immunology
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