Antibiotic Susceptibility Test
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What is antibiotics?
Compound that kill the bacteria
What is the disc-diffusion method?
Check Lab manual
What is zone of inhibition?
Check lab manual
What is the minimum inhibitory concentration(MIC)?
It is the least amount of a certain antibiotic that only inhibit the growth of the bacteria but not strong enough to kill the bacteria
What does Bacitracin(A disk) do?
Inhibits cell wall synthesis
What is the diameter of a zone of inhibition for us to conclude that the bacteria is susceptible to bacitracin?
Greater than 10 mm
Which type of bacteria is susceptible to Bacitracin? Be Specific
- Group A Beta-hemolysis Streptococci (GAS)-infection of skin and throats
- EX: Streptococcus pyogenes is sensitive to bacitracin while Streptococcus agalactiae is resistant
How does Novobiocin affect bacteria?
Interferes with ATP production
What is Novobiocin's susceptible diameter of the zone of inhibition?
Greater than 16mm
What does Novobiocin identify?
- Identify coagulase negative staphylococci
- EX: Staphylococcus saprophyticus
How does Optochin(O disk) intefere with bacteria growth?
Interfere with ATP production
What is Optochin's susceptibility diameter of zone of inhibition?
Greater than 14mm
Which type of bacteria does Optochin differentiate?
Differentiate Streptococcus pneumoniae from other Alpha hemolytic streptococci
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