Bile Esculin Hydrolysis test

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Author:
nghiatrinh1991
ID:
212494
Filename:
Bile Esculin Hydrolysis test
Updated:
2013-04-09 19:06:38
Tags:
bile Esculin hydrolysis test
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Description:
bile esculin
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  1. What is the differential ingredient in Bile Esculin Hydrolysis Test?
    Esculin
  2. What is the substrate of Bile Esculin Agar?
    glucose and esculetin
  3. Is the substrate easily hydrolyzed under acidic condition?
    Yes
  4. What does bacteria need to hydrolyze esculin?
    Bile
  5. What is the selective agent in Bile Esculin?
    Bile
  6. What is the indicator of iron?
    Ferric citrate
  7. Which bacteria group is bile esculin positive? Bile esculin negative?
    Enterococci

    Streptococcus pneumoniae and Streptococcus pyogenes
  8. Describe the process of a positive bile esculin test
    Bacteria can hydrolyze esculin and split it into esculetin. Esculetin combine with ferric citrate and form dark brown phenolic iron complex. Show up dark.
  9. Where can you find Enterococci, Enterococcus faecalis?
    Gastrointestinal tract, vagina, oral cavity
  10. Does Enterococci and Enterococcus faecalis need O2?
    NO, just area with nutrients
  11. Are Enterococci and Enterococcus faecalis highly resistant to antibiotic?
    Yes
  12. How do Enterococci and Enterococcus faecalis enter the blood stream?
    through invasive procedures such as catheters.

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