Oxidase Test

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Author:
nghiatrinh1991
ID:
212630
Filename:
Oxidase Test
Updated:
2013-04-10 08:37:28
Tags:
Oxidase Test
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Description:
Microbio lab oxidase test
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  1. What is the oxidase test purpose?
    To identify bacteria that contain cytochrome c oxidase
  2. What is the function of cytochrom C oxidase?
    Carry the electron from cytochrom C to O2 in Electron transport chain. reducing O2 to H2O

    Can also catalyze the reduction of cytochrome C by a chromogenic reducing agent
  3. Is Enterobacteriaceae oxidase (+) or (-)?
    Oxidase (-)
  4. What do we call chemical that develop color as they become oxidized?
    Chromogenic reducing agent
  5. What is the chromogenic reducing agent used in oxidase test?
    Tetramethyl-p-phenylenediamine(TMPD)
  6. What does it mean if the bacteria turn purple when TMPD is added to the bacteria?
    • The bacteria contain cytochrom c oxidase and that oxidized cytochrome c. 
    • The oxidase test is positive
  7. What happen when the bacteria is colorless when TMPD is added?
    Phenylenediamine is reduced

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