Prokaryotes- immediate production of transcription is mRNA
what type of RNA does polymeraseII produce?
what type of RNA does polymerase III produce?
mRNA genes and snRNA genes
what type of RNA does polymerase 4 produce?
5sRNA and tRNA genes
in alternate splicing what is different between the alternate splice products?
they differ in the choice of exons to be included in the mRNA
what are the 3 parts needed to specify the correct splicing of an intron?
5' consensus sequence
3' consensus sequence
the 3' end of an mRNA molecule correspons to the _____ terminus of the polypeptide it encodes
if you align a polypeptide sequence next to the gene that encodes it, which end of the polypeptide would line up closest to the promoter?
Define 3' UTR
UTR stands for untranslated region. untranslated regions are portions of the mRNA that are not translated
mRNA after the stop codon until the 3'end
mRNA between the 5'end and the start codon.
what part of tRNA forms base pairs with mRNA?
during translation amino acids are added to the ___ end of the growing polypeptide
suppose that you have isolated the DNA of a gene and want to predict what type of protein it encodes. is the sequence of the polypeptide encoded by the gene predicted by reading the DNA sequence of the coding strand or the non coding strand?
in an aminoacyl tRNA (charged tRNA) to what part of the tRNA is the amino acid attached?
at initiation of translation, in which site of the ribosome is the start codon located?
name and describe the difference between eukaryotic and prokaryotic translation
The eukaryotic ribosome needs the 5' cap and the polyA tail for assembly onto the mRNA
Eukaryotic mRNA have the kozak sequence surrounding start codon instead of Shine-delgarno
There is no f-met in eukaryotes
Translation occurs in separate compartment than transcription; it is in cytoplasm or on surface of endoplasmic reticulum