Micro Lab

Card Set Information

Author:
blondie94
ID:
215817
Filename:
Micro Lab
Updated:
2013-04-25 16:51:54
Tags:
Microbiology Nett IVCC Lab
Folders:

Description:
Microbiology Lab Notes
Show Answers:

Home > Flashcards > Print Preview

The flashcards below were created by user blondie94 on FreezingBlue Flashcards. What would you like to do?


  1. Naturally occurring compounds produced by bacteria and or fungi, that kill or inhibit other microbes.
    Antibiotics
  2. Many antibiotics ar eproduced by what two genera of bacteria and what genera of fungi?
    • Bacteria: Streptomyces, Bacillus
    • Fungi: Penicillum
  3. The antibiotic must harm the pathogen without harming the host cell.
    Selective toxicity
  4. How do antibiotics achieve selective toxicity?
    • Attacking the 70s ribosome
    • Attacking the cell wall
  5. What is the test called when testing different antibiotics?
    Kirby-Bauer Antibiotic Disk Sensitivity Test
  6. Least amount of an antibiotic that will inhibit the growth of a microbe.
    Minimum Inhibitory Concentrations
  7. What does MIC stand for?
    Minimum Inhibitory Concentrations
  8. MRSA
    Methicillin Resistant Staphylococcus aureus
  9. What does Vancomycin have to do with MRSA?
    Last line of defense against MRSA.
  10. What does the use of Vanco on MRSA result in?
    VRE (Vancomycin Resistant Enterococci)
  11. VRE
    Vancomycin Resistant Enterococci
  12. Exoenzyme
    Enzyme outside of the cell
  13. Endoenzyme
    Enzyme within the cell
  14. Name two exoenzymes.
    • Amylase
    • Lipase
  15. Name two endoenzymes.
    • Catalase
    • Cytochrome Oxidase
  16. Identify the equation for an Amylase Reaction.
    Starch ---Amylase---> Maltose + Glucose
  17. Identify the equation for a Lipase Reaction.
    Lipids ---Lipase---> Fatty Acids + Glycerol
  18. Identify the equation for a Catalase Reaction.
    2 H2O2 ---Catalase---> 2 H2O + O2
  19. Identify the equation for a Cytochrome oxidase reaction.
    2H + 2e- + 1/2 O2 ---Cytochrome oxidase---> H2O
  20. A Lipase test is done on a Spirit Blue Agar Plate, what happens if Lipase is present (positive)?
    A clear zone surrounds the bacteria
  21. An Amylase test is done on a Starch Agar Plate, what happens if Amylase is present (positive)?
    A clear zone surrounds the bacteria
  22. What test is performed to see if catalse is present?
    Hydrogen Peroxide is dropped on bacteria, effervescense occurs when positive. (Bubbling)
  23. What test is performed to see if Cytochrome Oxidase is present?
    Bacteria is placed on a Cytochorome Oxidase disc and water is then added to activate the test. Disc turns purple when positive.
  24. Enzymes that attack Amino Acids
    Amino Acid Enzymes
  25. Name three Amino Acid enzymes?
    • Cysteine desulfhydrase
    • Lysine decarboxylase
    • Phenylalanine deaminase
  26. What does Cysteine desulfhydrase do to an amino acid?
    Removes Desulfhydrase bonds in an amino acid.
  27. What does Lysine decarboxylase do?
    Removes the carboxyl group of an amino acid.
  28. What does Phenylalanine deaminase do?
    Removes the amino group of an amino acid.
  29. What type of material is used to test for Cysteine desulfhydrase?
    Peptone Iron Agar Deep
  30. What is the equation for cysteine desulfhydrase?
    Cysteine --Cysteine desulfhydrase--> H2S
  31. When a Peptone Iron Agar Deep is positive for cysteine desulfhydrase, what happens?
    Agar turns black from iron
  32. What is the equation for Lysine decarboxylase?
    Lysine --Lysine decarboxylase--> Cadaverine + CO2
  33. What two conditions are necessary for Lysine decarboxylase?
    • Acidic
    • Anaerobic
  34. Explain the process when testing for Lysine decarboxylase.
    Lysine is put in test tube with a ph indicator and Durham tube to catch the gas. Mineral oil is placed on top of fluid to maintain an anaerobic condition.
  35. Mini inverted tube to capture gas in a Lysine decarboxylase test.
    Durham tube.
  36. What is the purpose of mineral oil in a Lysine decarboxylase test?
    Maintain an anaerobic environment.
  37. How do you know when the test for Lysine decarboxylase is positive?
    Purple with gas bubble in durham tube.
  38. What is the equation for Phenylanine deaminase?
    Phenlalanine --Phenylalanine deaminase--> Phenylpyruvic acid
  39. How do you know when the test for Phenylanine deaminase is positive?
    When Ferric Chloride and Hydrocloric acid is put in tube, turns green
  40. Name three enzymes that attack "N" substrates.
    • Urease
    • Gelatinase
    • Nitrate Reductase
  41. What is the equation for Urease test?
    Urea + H2O --Urease--> Ammonia + CO2
  42. What is contained in Urea Broth?
    Urea + pH indicator
  43. How do you know when the test for Urease is postive?
    Turns Pink
  44. What is the equation for a Gelatinase test?
    Gelatin (solid) --Gelatinase--> Gelatin (liquid)
  45. What type of agar is used to test for gelatinase?
    Tryptic Soy Gelatin Agar
  46. How do you know when the test for Gelatinase is positive?
    Liquid
  47. What is the equation for Nitrate Reductase?
    Nitrate --Nitrate Reductase--> Nitrite + H2O
  48. What type of broth is used for the Nitrate Reductase test?
    Nitrate Broth
  49. How do you know if Nitrate Reductase is positive?
    • After adding Nitrate A&B, red is positve
    • After adding Zinc, red is negative
  50. Give three examples of a monosaccharide.
    • Glucose
    • Fructose
    • Galactose
  51. Give three examples of a disaccharide.
    Sucrose, Maltose, Lactose
  52. Give three examples of a polysaccharide.
    • Starch
    • Glycogen
    • Agar
  53. In a test for fermentation of a carbohydrate, what is present?
    Acid and Gas
  54. What type of agar is used to test for fermentation of a carbohydrate?
    Phenol Red Carbohydrate Broth
  55. What is the pH indicator for Phenol Red Carbohydrate broth?
    • Phenol Red
    • Basic- Pink
    • Neutral - Red
    • Acid - Yellow
  56. What is used to capture the gas when testing for fermentation of a carbohydrate?
    Durham tube.
  57. Enzymes that attack RBCs.
    Hemolysins
  58. Secretes hemolysins that cause incomplete lysins of RBC's.
    Alpha hemolytic Streptococci
  59. Secretes hemolysins that cause complete lysins of RBC's.
    Beta hemolytic Streptococci
  60. Secretes no hemolysins.
    Gamma hemolytic streptococci

What would you like to do?

Home > Flashcards > Print Preview