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Bio 3000 - Transcription in Eukaryotes
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In what way is Eukaryotic Transcription more complex than Bacteria?
More Initiation Factors.
What is the Eukaryote Aminoacyl tRNA?
Met-tRNA
i
met
Do eukaryotes have Shine-Delgarno sequences?
No
What is required for binding of the small (40s) subunit?
Initiation Factors
Met-tRNA
i
met
GTP
What is the Scanning Model in Eukaryotic Translation?
When the 40s subunit "scans" the mRNA for a "start" codon.
What makes a good start codon?
Optimal Sequence
5'- ACC
A
UGG -3'
Purine at -3
Guanine at +4
What does the +1 position lie in an optimal start sequence?
On the bolded A in,
5' ACC
A
UGG -3'
(First base in the traditional AUG start)
What are Kozak's rules?
Rules defining an optimal start codon.
Optimal Sequence
A
CC
A
UG
G
Purine at -3
G at +4
What are the Two main eukaryote inition factors?
eIF2
eIF4F
What is the structure of eIF2?
Three subunits; α, β, γ.
What is the structure of eIF4F?
Composed of other initiation factors
eIF4A
eIF4G
eIF4E
What is the role of eIF2?
To bring initiator met-tRNA
i
met
to the 40S subunit.
Uses GTP
What is the role of GTP in initiation?
To aid eIF2 in bringing the first tRNA
What is the CAP-Binding complex?
Essentially eIF4F
The combined eIF4A, eIF4G and eIF4E subunits.
What is the role of the CAP binding complex?
Binding the mRNA
What is the effect of Phosphorylating eIF2 (α subunit)?
It binds tightly to eIF28, preventing further initiation.
Author
Ant
ID
215828
Card Set
Bio 3000 - Transcription in Eukaryotes
Description
Bio 3000
Updated
4/25/2013, 11:11:25 PM
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