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In what way is Eukaryotic Transcription more complex than Bacteria?
More Initiation Factors.
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What is the Eukaryote Aminoacyl tRNA?
Met-tRNAimet
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Do eukaryotes have Shine-Delgarno sequences?
No
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What is required for binding of the small (40s) subunit?
- Initiation Factors
- Met-tRNAimetGTP
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What is the Scanning Model in Eukaryotic Translation?
When the 40s subunit "scans" the mRNA for a "start" codon.
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What makes a good start codon?
- Optimal Sequence
- 5'- ACCAUGG -3'
- Purine at -3
- Guanine at +4
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What does the +1 position lie in an optimal start sequence?
- On the bolded A in,
- 5' ACCAUGG -3'
- (First base in the traditional AUG start)
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What are Kozak's rules?
- Rules defining an optimal start codon.
- Optimal Sequence
- ACCAUGG
- Purine at -3
- G at +4
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What are the Two main eukaryote inition factors?
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What is the structure of eIF2?
Three subunits; α, β, γ.
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What is the structure of eIF4F?
- Composed of other initiation factors
- eIF4A
- eIF4G
- eIF4E
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What is the role of eIF2?
- To bring initiator met-tRNAimet to the 40S subunit.
- Uses GTP
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What is the role of GTP in initiation?
To aid eIF2 in bringing the first tRNA
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What is the CAP-Binding complex?
- Essentially eIF4F
- The combined eIF4A, eIF4G and eIF4E subunits.
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What is the role of the CAP binding complex?
Binding the mRNA
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What is the effect of Phosphorylating eIF2 (α subunit)?
It binds tightly to eIF28, preventing further initiation.
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