Bio 3000 - Introduction to Splicing
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How is Protein Synthesis controlled?
- Transcription Levels
- Production of Mature mRNA
- mRNA Stability (Turnover)
- Regulation of Translation
What are the Three types of RNA alteration after Transcription?
- Covalent alterations (Capping, Poly-A)
- Removal of Introns
- Changing Sequences or Nucleotide Structure
Why can Bacteria engage in Simultaneous Translation/Transcription?
Bacteria have no barriers separating Transcription and Translation.
When does eukaryotic mRNA leave the nucleus?
After it has completed processing.
Do tRNA's have introns?
No, or no more than one.
Do mRNA's have introns?
Yes, anywhere from 0-362
Why is it impotant to remove Introns accurately?
If introms are not occur, different translation (frame-shift) may occur.
What are the 5' and 3' splicing signals found in nuclear mRNA?
5'- --- GU ---- --A- ---- AG --- -3'
Are AG and UG the only consenses sequences for splicing?
No, they are just motifs. Often the consensus sequences associated extend beyond the GU and AG.
Whay are the Mammalian Splicing Consensus Sequences?
5'- ---AG/GUrAGU --- --- A --- -y- yAG/G--- -3'
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