In the paper by Xia et al. 2011, one of the final conclusions of the paper was that they believed they identified an effective anti-HIV siRNA gene to target viral RNA. Do you think their data supported this conclusion, why or why not? If not, what would you do to provide further experimental evidence?
- The data showed that in cells treated with antiHIV adenovirus, p24 expression remained at a low level, while it increased with untransduced cells
- HOWEVER, the data did not show a control using a copy of the adenovirus without CCR5 siRNA gene removed. This would help to see if CCR5 siRNA was directly responsible for this reduction of p24 expression, or if it was another portion of this plasmid that was transduced.