1. What happens when T state switches to R state and vice versa?
2. What causes the Bohr Effect?
3. What does increasing acidity do to p50? Curve? Affinity for O2? Bohr protons? Ion pairs? why? What state is stabliized?
3.5 Do O2 and H+ bind in same places?
4. Draw picture of increasing pH effects on dissociation curve.
1. Large scale conformational changes, new interactions are made and old ones are broken to stabilize one state over the other based on O2 levels.
2. Effect of pH and [CO2] on Hb binding to O2.
3. Increasing acidity causes formation of salt bridges (b/c things get protonated
p50 increases (b/c affinity decreases), curve shifts right, affinity for O2 decreases, Bohr protons are there (protonated) forming ion pairs promoting tense state (stabilizing deoxyHb) to increase release of O2 to tissues
High acidity signals low O2: CO2 ratio, signaling the need for the release of O2.
T state - relaxed state.