1. How does cytochrome p450 create ROS? Which one is created? What does it have to react /w first?
2. How is hydroxyl radical created in human body? What is it responsible for?
2.5 What are normal functions of NO? (5)
3. How is .NO produced? (enzyme, what is NO, what are the 3 types of enzymes involved?
4. Which enzyme is highly regulated? What cofactors are needed (5)
1. By reacting with oxygen to create superoxide radical. Xenobiotic agents can be metaebolized to radical species (i.e., aflatoxin & other foods)
2. Fenton rxn. SIGNIFICANT PART OF DAMAGE ASSOCIATED w/ OXIDATIVE STRESS
2.5 Vascular tone, insulin secretion, airway tone, cardiac function, and angiogenesis
3. By nitric oxide synthase (nNOS - neuronal, iNOS - inducible, and eNOS - endothelial) NO is a signaling molcule
4. NOS and requires 5 co-factors (FAD, FMN, heme bound O2, NADPH, and BH4).