Isolation

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Author:
nghiatrinh1991
ID:
218211
Filename:
Isolation
Updated:
2013-05-07 01:56:53
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Isolation
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Description:
Isolation
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The flashcards below were created by user nghiatrinh1991 on FreezingBlue Flashcards. What would you like to do?


  1. When do we use isolation?
    To obtain a pure culture from a mix culture
  2. What is a colony?
    It is a visible mass of cells produced on culture media from a single cell or single CFU(colony forming unit)
  3. Why do we use the term CFU?
    Because colony can be form by 1 cell or many cell so we just call 1 of those mass as a unit that form colony
  4. What is the benefit of the streak plate? What is the disadvantage?
    • Allow to get individual colonies, and does not require serial dilution.
    • the disadvantage is that you can't quantify bacteria
  5. What is the spread plate method use for?
    to get individual colonies, good for quantification, and accompanied by serial dilution
  6. Where do you expect contamination if some microbes were to get in?
    Anywhere away from the streak or if they have different morphologies
  7. What is a selective media?
    A media that inhibits or suppresses unwanted microbes but favors and encourages the growth of desired microbes
  8. What is differential media?
    • Substances or indicators built in the media that allow to distinguish certain type of bacteria. 
    • Allow multiple type of bacteria to grow but only the wanted one are visible

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