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- Large GPB with spores
- (only spore forming bacteria that grows aerobically)
- Spores oval in shape - do not swell beyond cell
- May chain to resemble bamboo rods
- sometimes V & L shaped
Growth on media for both types of bacillus spp.
SBA - 18-24 hours
- anthracis: gray white frosted medusa head projections coming up
- cereus: beta hemolysis, gray white frosted
Catalase test for both bacillus spp
Motility for both species
Antibiotic therapy for both bacillus spp.
anthracis: Sensitive to penicillins
- cereus: flouroquinalones, tetracylcines and others.
- for cereus: avoid beta lactams such as penicillins and cephalosporins they are less effective.
Clinical specimen for anthracis
blood, CSF, etc
What are the confirmatory identification for anthracis?
- Phage lysis
- Capsule production on bicarbonate media
- DFA (capsule antigen cell wall.
- PCR - specific ID of DNA
- India ink stain
- McFadyean stain
B cereus is most noted for what type of infection?
Food poisoning - Is toxin mediated which is food that is contaminated with bacteria.
Less common: opportunisitic infection
Epidemiology/etiology for B. anthracis
- Typically acquired through contact with anthrax-infected animals or animal products.
- Cutaneous - direct contact - wool sorters disease.
- Inhalation - aspiration of spore aerosol
- Gastrointestinal: eating of contaminated meat.
NOT PERSON TO PERSON SPREAD
Epidemiology for B. cereus
- Diarrheal - meat 24 hrs self limiting
- Emetic - fried rice 10 hrs self limiting
- Both caused by 2 distinct toxins
Opportunistic: ocular infection & wound infection