Listeria species

Card Set Information

Author:
ucstudent01
ID:
219230
Filename:
Listeria species
Updated:
2013-05-12 12:54:48
Tags:
listeria
Folders:

Description:
listeria
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  1. Gram stain appearance
    • GP short rods or coccobacilli
    • NOT ACID FAST
  2. Source of specimen
    • Blood
    • CSF
    • Amniotic fluid
    • Genital tract
    • Stool
    • Respiratory
    • etc..
  3. Media description
    • SBA - 18-24hrs
    • small translucent gray
    • Narrow zone of Beta hemolyisis

    If dealing with mixed flora, cold enrichment at 4C is useful since this will grow at colder temps.
  4. Biochemical profile
    • Positive:
    • Catalase
    • Motility 
    • Umbrella motility Agar at 25C
    • Camp
    • Esculine hydrolysis
    • Negative:
    • H2S
  5. Where is L. monocytogenes found?
    • found widespread in nature, decaying vegetation, but carried by numerous humans and animals.
    • concern: Easy access to food processing
  6. Epidemiology
    • Disease: Listeriosis
    •         Predominately a food borne illness
    • Cutaneous: occupational exposure
    •         Meat processors and veterinarians
    • High Mortality
    • Those mostly susceptible are immuno- compromised
  7. Diagnosis
    • Recognition of aerobic GPB
    •   ~ Beta hemolytic (clear zone)
    •   ~ Biochemical profile
    •   ~ Motility characteristics
    •   ~ Growth at 4C

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