Micro lab quiz 5 part two

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Micro lab quiz 5 part two
2013-05-16 01:29:25
micro lab

covers labs 12-13
Show Answers:

  1. Methyl Red tests for what?
    positive result?
    • organisms capable of performing a mixed acid fermentation
    • -overcomes phosphate buffer in medium to lower pH
    • -Methyl red is red at pH 4.4., yellow at 6.2
    • Positive = red (ferments acid)
    • Negative = yellow
    • orange = negative/inconclusive
  2. Voges-Proskauer Test tests for what?
    positive result?
    • test for ability to ferment glucose and quickly convert acid products to acetoin and 2,3-butanediol
    • positive result: red
    • negative: no color change
  3. What is the "clean catch"?
    Pee a little bit to flush out bacteria on skin, stop, then catch rest of urine in cup
  4. What is a urinalysis?
    Analysis of urine by physical, chemical, and microscopical means to test for the presence of disease, drugs, etc.
  5. What information does a urinalysis dipstick provide?
    • glucose
    • bilirubin
    • ketone
    • specific gravity
    • blood
    • pH
    • protein
    • urobilinogen
    • nitrite
    • leukocytes
  6. What is the purpose of the coagulase test?
    positive result?
    differentiate staphylococcus aureus

    • add organism to plasma on slide
    • positive: agglutination in 2 minutes
  7. What is your blood type based on?

    What is present if you have type A blood?

    Type AB?

    Type O?
    Which antigens are present/what antibodies are in the plasma

    A: A antigens, B antibodies in plasma

    AB: A and B antigens, no antibodies (universal receiver)

    O: No antigens, A and B antibodies (universal donor)
  8. What is the universal donor?  receiver?
    Donor: O-

    Receiver: AB+
  9. what is the RH factor?
    An antigen present on the blood cell (RH+)

    • Someone who is RH+ can get RH+ or RH- blood
    • Someone who is RH- can develop RH antibodies after receiving a transfusion of RH+ blood
  10. How do you determine someone's blood type in lab?
    Each well has either A antibodies, B antibodies or RH antibodies

    If a well agglutinates, that antigen is present (i.e. if the well with A antibodies agglutinates, A antigens are present and they are sticking to the antibodies- which are not normally present in type A blood- therefor it is type A blood)

    If it clumps, that's what type it is