BactPhysPresentationQuestion

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victimsofadown
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220926
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BactPhysPresentationQuestion
Updated:
2013-05-24 03:22:38
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BactPhysPre
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BactPhysPre
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  1. What is the most studied model organism of Streptomyces?
    S. coelicolor A3 (2)
  2. What protein was identified to be bound to NdgR?
    metH
  3. What is the metabolic target of NgdR in this study?
    methionine biosynthesis
  4. What are TLRs?
    Toll-like receptors
  5. How long were the mice fasted for?
    14 hours
  6. What type of cells were used as receptors?
    HEK 293 (human embryonic kidney cells)
  7. Name one of three genes affected by CO2 levels in S. pnuemoniae
    folC
  8. Did folic acid supplementation help the ΔfolC strain?
    No, it did not
  9. Name a limitation to Tn-seq
    Defects could be covered up by Tn-seq reading from a different defect/mutation
  10. What are some symptoms of CDI?
    Diarrhea, Pseudomembranous colitis, inflammation
  11. What are the two purposes of this paper?
    • Identify surface/regulator proteins required for biofilm formation
    • Find role of biofilm to resistance of antibiotics
  12. What is the main role of sleC?
    Germination of spores (may hydrolyze peptidoglycan in vegetative cells)
  13. Why are gram negative bacteria used in OMV studies?
    The outer membrane vesicles form from the outer membrane which is not present in gram positive bacteria
  14. Can Pseudomonas aeruginosa form OMVs without the presence of PQS?
    No, PQS is required for OMV formation
  15. What was the significance of the results?
    Previous experimentation showed only that a lack of peptidoglycan-bound proteins caused OMV formation.  This study introduced a new mechanism for their formation with the investigation of PQS
  16. What does the "Cas" from CRISPR/Cas stand for?
    CRISPR associated proteins
  17. What technique did they use that was not covered in class?
    Recombineering
  18. How many phages were inhibited by the CRISPR/Cas adaptive immune system
    6

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