Lab 12 study guide

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busdriverre
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2227
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Lab 12 study guide
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2009-12-04 11:35:44
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Lab 12 Bacteriophages
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Lab 12 study guide
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  1. define bacteriophage
    bacterial viruses
  2. define genome
    a complete set of a virus's hereditary material
  3. define proliferate
    to grow or produce by multiplication of parts
  4. define plaque
    The lysis of bacteria growing on an agar plate produces clearing in the lawn of bacteria. These clearing are called plaques.
  5. define plaque forming unit (PFU)
    Since one infectious particle is responsible for one visible plaque, each particle is considered to be a plaque forming unit.
  6. define host
    the cell the bacteria infects
  7. define T4 phage
    • bacteriophage that looks like a spaceship
    • DNA is packaged in the head and the tailhelps with the infection process
  8. what is a nucleic acid?
    They carry the genetic information in all cells.
  9. Which types of nucleic acid may viruses contain?
    Viruses carry either RNA or DNA but never both. They are designated as either RNA or DNA viruses.
  10. Can a virus contain more than one type of nucleic acid?
    No. It is either an RNA or a DNA virus, but never both.
  11. What are the 5 steps of the T4 phage life cycle?
    • adsorption
    • penetration
    • replication
    • assembly
    • release
  12. What is the adsorption phase?
    tail fibers of the phage bind to the host's cell wall (attachment)
  13. What is the penetration phase?
    The enzymes present in the phage tail degrade the host cell wall and the viral nucleic acid passes through the hollow core into the host cel's cytoplasm.
  14. What is the replication phase?
    the phage genome uses the cell's synthetic machinery for production of new phage components.
  15. What is the assembly phase?
    the phage components are assembled into mature virulent phage particles
  16. What is the release phase?
    enzymes lyse the host cell wall and allow the viruses to escape from the host
  17. What is the host of the T4 bacteriophage?
    E. coli
  18. Which component of the T4 bacteriophage contains its nucleic acid?
    DNA is packaged in the head
  19. What is the purpose of 5 tryptone agar plates?
    They are used to plate the dilution tubes
  20. What is the purpose of eight 9-mL tryptone broths?
    They are used for the dilution process
  21. What is the purpose of the broth culture of E. coli?
    The E. coli is the host for the T4 bacteriophage
  22. What is the purpose of the sterile 1 mL pipettes?
    A sterile pipette must be used each time to accurately transfer the broth and not cross contaminate

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