gram stain limitations.txt

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nmeumonic?

These Rascals May Microscopically Lack Color
Which don't stain well?
- Treponema
- Rickettsia
- Mycobacteria
- Mycoplasma
- Legionella pnewnophila
- Chlamydia
Why does Treponema not stain well?

(too thin to be visualized)
Why does Rickettsia not stain well?

intracellular parasite
Why does Mycobacteria not stain well?
- high lipid content in cell wall
- detected by carbolfuchsin i n acid-fast stain
Why does Mycoplasma not stain well?

( no cell wall)
Why does Legionella pneumophila not stain well?

(primarily intracellular)
Why does Chlamydia not stain well?
- intracellular parasite ; lacks
- muramic acid in cell wall)