Card Set Information
Pentose Phosphate Pathway and NADPH
Pentose phosphate pathway (PPP)
Provides route for metabolism of glucose
ATP is consumed/produced
production of reducing power in form of NADPH and ribose
mammary glands, adipose tissue, adrenal cortex
: generate NADPH for biosynthetic rxns ex: fatty acid/cholesterol synthesis
production of 5 carbon
generates ribose 5 phosphate & NADPH for nucleotide biosynthesis. one can get either one, or both.
regulated by G6PD
2 Pentose Phosphate Pathways
1. Irreversible oxidative.
2. Reversible non-oxidative.
Irreversable oxidative rxns
convert G5P to Ribulose5P w/ release of Co2 and nadph
PPP in RBC
NADPH used to regenerate the antioxidant glutathione, which protects cells against damage. (keeps glutathione reduced)
Nadp is it's coenzyme
Rate limiting enzyme
converts g6p to 6phos-glu-nolacetone. Final is (ribulose5p)
inhibited by NADPH
Ratio of NADPH/NADP is high which inhibits enzyme activity.
In enhanced activity, ratio decrease, and demand of NADPH goes up.
2 nadph for biosynthesis & glutathion (GSH)
Insulin regulates G6PD, pathway increases in FED state.
high energy, used for reductive synthetic reaction.
competitive inhibitor of enzyme G6PD
Antioxidant, neutralizes the oxidant activity of H2O2.
tranfer electrons to electron transport chain to make ATP
6phos-glu-nolactone hydrolyzed by 6phos-glu-nolactone