Micro Lab 2

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MeganM
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225803
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Micro Lab 2
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2013-07-07 23:12:47
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Micro
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Lab Exam 2
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  1. Skim milk agar is a test for a _______ type enzyme.
    protease
  2. The protein in skim milk is _______ and a positive test for its digestion is _______.
    casein; casease
  3. This plate is used to test for the type of digestive enzyme called ________ because it breaks down _______  to ___________. 
    lipase; lipids; fatty acids & glycerol.
  4. This slide is test is used to detect the enzyme _______ .  The  substrate is ________ and the end product is _______. 
    catalase; H2O2; O2


  5. Which gram positive coccus produces a negative result for this test? Which is positive?
    Strep; Staph
  6. What is the indicator in this media?
    What color is it if it is "acid"?
    Phenyl Red; yellow
  7. What type of end product does fermentation produce? What color does it change the indicator to?
    acid; yellow


  8. The small tube in the broth is called a ________ and will collect _______ produced in fermentation reactions.
    fermentation tube; gas
  9. Gelatin is a ______ derived from animals. This test detects the enzyme ______ that digests the material.
    protein; gelatinase or protease


  10. A positive nutrient gelatin test is described as _______ after inocculation & incubation & chilling in the refrigerator. Which tube below is (-) for the digestive enzyme?
    liquid; B & C
  11. In the  starch test, the name of the starch used is ______ and the enzyme we are testing for is called ________.
    amylose; amylase
  12. In a starch test, the end product of starch hydrolysis is _______.
    glucose
  13. What reagent is added after incubation to see the positive test in starch hydrolysis?
    iodine
  14. In this test, E. coli is (+) or (-) for starch hydrolysis? Why?
    negative; no ZOC
  15. The indicator in a urease test is ________ and is ______ if positive.
    phenyl red; hot pink
  16. Describe the positive starch test.
    ZOC
  17. The pH of a positive urease test is ________.
    alkaline
  18. Urea is produced by the _________ .
    deamination of amino acids that make up proteins
  19. Microbes can degrade urea. What are the two end products of the digestion of urea?
    NH3 (ammonia) and CO2 (carbon dioxide)
  20. What is a defined media? What is an example of one?
    • It means we know exactly what is in it;
    • Simmons citrate media
  21. The only source of carbon in simmons citrate media is ________.
    citric acid (or citrate)
  22. A positive test in simmons citrate media is described as ______.
    blue
  23. What does a positive simmons citrate test mean?
    The organism produces the enzyme citrate permease and can use citric acid as the SOLE source of carbon.
  24. What is the indicator in a simmons citrate test?
    What is the pH of the positive tube?
    • BTB;
    • alkaline
  25. Which tube is (+) for MR? WHat's the name of the indicator added after innoculation and incubation?
    • B (b/c its red)
    • Methyl Red
  26. What is the pH of the positive MR test?
    acidic
  27. What enzyme system is being tested here?
    Nitrate reductase
  28. Name 3 end products of the nitrate reduction test.
    • Ammonia (NH3)
    • Nitrogen gas
    • Nitrite
  29. What is the sugar in high concentration in the MR-VP media?
    glucose
  30. Describe the positive VP test.
    red
  31. What is the name of the reagents added after innoculation and incubation in the VP test?
    Barrit's A & B
  32. Name one coliform enteric that is positive for VP.
    E. Cloachae (see flow chart)
  33. The MR-VP tests are part of the ______ battery of tests to separate coliforms into two categories for further testing and identification.
    IMVC
  34. What are the 3 tests for SIM media?
    sulfur, Indole, motility
  35. In SIM media, what color is a positive for "s" ?
    black
  36. What is added to the SIM media?
    Iron
  37. Iron is added to the SIM media to react with _______ produced from the breakdown of _____ containing amino acids.
    • Hydrogen Sulfide (H2S)
    • sulfur
  38. A positive "I" is a _______ color after the addition of _______ in SIM media.
    • red ring;
    • kovacs
  39. The red ring color in SIM media after adding kovacs is the result of the break down of what?
    the amino acid tryptophan
  40. A positive "M" in SIM media is described as what?
    fuzzy
  41. A SIM media is innoculated with a _____ because it is a _____.
    • needle;
    • agar deep
  42. The "base" medium in decarboxylase media is _______.
    Moeller
  43. The indicator in decarboxylase media.
    BCP
  44. In a decarboxylase test, a (+) test is ______ and indicates an ______ pH.
    • purple;
    • alkaline
  45. What is added to decarboxylase test media after innoculation and BEFORE incubation and why?
    mineral oil to seal out the air
  46. TSI stands for?
    Triple Sugar Iron
  47. The 3 sugars in TSI.
    glucose, sucrose, lactose
  48. How much glucose is in TSI compared to sucrose & lactose?
    1/10 as much
  49. The indicator in TSI media.
    phenyl red
  50. Phenyl red in TSI media indicates _________ end products when the color is YELLOW.
    acid fermentation
  51. Which tube is positive for glucose fermentation only? Why?
    • The 2nd one from the left;
    • acid butt (yellow) but has an alkaline slant
  52. What pathogen would produce an acid butt (yellow) and alkaline slant (red) in TSI media?
    shigella :(
  53. Which TSI tube indicates hydrogen sulfide production?
    The 3rd from left because it's black
  54. What is added to TSI media to make the black color show up?
    iron
  55. Which tube is showing lactose fermentation (TSI  media)? Why?
    1st on left - because it is acid-acid & has gas at the bottom
  56. Which tube is showing no fermentation (TSI media)? Why?
    • One on far right - it's red.
    • (Pseudomonia)
  57. Phenylalanine is an _______.
    amino acid
  58. What is the reagent added in phenylalanine deaminase that is added after innoculation & incubation to see a positive deaminase rxn?

    FeCl3
  59. The positive phenylalanine deaminase test is a ______ color.
    green
  60. The end product of phenylalanine deaminase is __________.
    Pheynol pyruvic acid
  61. DNASE plates test for the presence of what enzyme?
    nuclease
  62. The indicator in a DNASE plate is ________ and a positive DNASE is described as a ______.
    • methyl green;
    • ZOC
  63. A pathogen that is positive for DNASES.
    Staphlococcus aureus
  64. What two enterics would be (+) for hydrogen sulfide in a TSI test?
    salmonella & proteus
  65. Which specific other test would you run to separate salmonella & proteus?
    urease test
  66. When running a urease test to separate salmonella & proteus, what color would a (+) test be?
    hot pink

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