19. The papers covered in the lecture on asymmetric cell division followed the behavior of mother versus daughter centrosomes in various cell types. For the following proteins indicate which are specifically associated with the mother centrosome/centriole and which associate only/primarily with the daughter. If some associate with both equally indicate that as well. Where known, also indicate the function(s) of the proteins from this list.
- a. a. NuMA (Nuclear mitotic apparatus protein): Fxn in interphase in nucleus, mitosis in spindle poles and at cortex, especially in late metaphase-telophase. (In ACD, the daughter centrosome with NEW mother centriole is inherited to the daughter cell with NuMA preferentially localized to the cell cortex, whereas the daughter centrosome with the NEW mother centriole migrates to the other daughter cell. Izumi and Kaneko et.al. During SCD, the daughter centrosome with the old mother centriole migrates to the daughter cell with NuMA localized to the cell cortex.)
- b. ?-tubulin: primary component of PCM, and is centrosome specific. Aids in helping to form template of MTs, along with gamma-TuRC.
- c. ODF2/cenexin : OLD MOTHER (mostly) centrosome marker, protein acquired by new mother during maturation (leading up to the G2/M transition), but amount of staining GREATER for the old mother relative to the new mother centriole.
- d. Centrobin: DAUGHTER cell marker. Necessary for daughter centrioles to keep PCM and MTOC activity during Interphase in NB cells. Phosphorylation required of CNB to work. Also controls mother-daughter centriole Asymmetry in Drosophila neuroblasts (Januschke et al. 2013)
- e. Ninein: MOTHER centiole marker. Fxn in positioning and anchoring microtubules minus ends in epithelial cells. It concentrates at the appendages surrounding the mother centrioles and the microtubule minus ends.
- f. Centrin 1: MOTHER and DAUGHTER associated, each centriole contains Centrin protein. Required for DUPLICATION of centrioles. This component of centriole, found in centrosome of eukaryotes