Card Set Information
microscopes depend on what 3 factors?
magnification, contrast, resolution
a compound microscope has _______lenses which are the ________and _______lens.
2, ocular & objective
With a typical compund microscope, the objective lens that will provide the largest, overall field of view is _____ for a total magnification of 40x.
What is the total magnification of the ocular lens (10x) and the oil immersion lens (100x)?
Most cell structures in on e microorganism are 3-5 nanometers apart. The detailed intracellular makeup of the microbe an be studied with the _____(sem or tem?)
stains increase contrast so you can see the microorganism
What are mordants?
not dyes, but intensify stain by increasing a cell's affinity for dye
Stains are grouped into what 3 classes?
simple, differential, special
What type of stain is a basic dye used to make cells visible?
What are the 3 steps used in differential stains?
primary stain, destaining, counterstaining
Counterstaining is used to reveal the cell or parts of cells that have been _____
What 2 stains are used extensively in micro?
gram stain and acid fast stain
Who developed the Gram stain?
What is the primary stain used in Gram stain?
What is the mordant used in a Gram stain?
What is the counterstain used in a Gram stain?
After the 4 steps of the Gram stain are applied, a bacterium has a deep violet color, this is because it retained Genetial violet and safranin and is Gram______
A bacterium retains sfranin after decolorizing agent removes previously applied genetian violet of the Gram stain. The bacterium is Gram
Acid-fast stains are especially important test for the genus
the primary stain for the acid fast stain is
the counterstain for the acid fast stain is
A petri dish is used to hold the nutrient
Coliform bacteria makes acid from ___and turns neutral red
Blood agar is a _________growth medium.
The purpose of serial dilution is to reduce the number of bacteria for counting purposes and for viewing colony
A pure culture consists of a _______ type of microorganism.
The requirement for sterilizing most microorganisms by moist heat is _______for ________.
121*C for 15-20 mins
Tuberculosis is caused by the genus