micro

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Author:
charflynt1
ID:
23092
Filename:
micro
Updated:
2010-06-10 22:55:39
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ch3
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review
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  1. microscopes depend on what 3 factors?
    magnification, contrast, resolution
  2. a compound microscope has _______lenses which are the ________and _______lens.
    2, ocular & objective
  3. With a typical compund microscope, the objective lens that will provide the largest, overall field of view is _____ for a total magnification of 40x.
    4
  4. What is the total magnification of the ocular lens (10x) and the oil immersion lens (100x)?
    1000x
  5. Most cell structures in on e microorganism are 3-5 nanometers apart. The detailed intracellular makeup of the microbe an be studied with the _____(sem or tem?)
    TEM
  6. stains increase contrast so you can see the microorganism
    better
  7. What are mordants?
    not dyes, but intensify stain by increasing a cell's affinity for dye
  8. Stains are grouped into what 3 classes?
    simple, differential, special
  9. What type of stain is a basic dye used to make cells visible?
    simple
  10. What are the 3 steps used in differential stains?
    primary stain, destaining, counterstaining
  11. Counterstaining is used to reveal the cell or parts of cells that have been _____
    destained
  12. What 2 stains are used extensively in micro?
    gram stain and acid fast stain
  13. Who developed the Gram stain?
    Christian Gram
  14. What is the primary stain used in Gram stain?
    crystal violet
  15. What is the mordant used in a Gram stain?
    iodine
  16. What is the counterstain used in a Gram stain?
    Saffranin
  17. After the 4 steps of the Gram stain are applied, a bacterium has a deep violet color, this is because it retained Genetial violet and safranin and is Gram______
    positive
  18. A bacterium retains sfranin after decolorizing agent removes previously applied genetian violet of the Gram stain. The bacterium is Gram
    negative
  19. Acid-fast stains are especially important test for the genus
    mycobacterium
  20. the primary stain for the acid fast stain is
    carbol fuchsin
  21. the counterstain for the acid fast stain is
    methylene blue
  22. A petri dish is used to hold the nutrient
    agar
  23. Coliform bacteria makes acid from ___and turns neutral red
    lactose
  24. Blood agar is a _________growth medium.
    ?
  25. The purpose of serial dilution is to reduce the number of bacteria for counting purposes and for viewing colony
    __________?
  26. A pure culture consists of a _______ type of microorganism.
    single
  27. The requirement for sterilizing most microorganisms by moist heat is _______for ________.
    121*C for 15-20 mins
  28. Tuberculosis is caused by the genus
    m. tuberculosis

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