Micro chap4

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Anonymous
ID:
231745
Filename:
Micro chap4
Updated:
2013-08-28 20:11:04
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Micro Chapter
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Micro Chapter 4
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  1. General Principals of Microscopy?
    • Wavelength of radiation
    • Magnification
    • Resolution
    • Contrast
  2. Difference in intensity between two objects, or between an object and background, Important in determining resolution, Staining increases it, The use of light that is in phase also increases it?
    Contrast
  3. Bright-field microscope, Dark-field microscope, Phase microscope, Fluorescent microscope, Confocal microscope?
    Light Microscopy
  4. Best for observing pale objects, Only those light rays scattered by specimens enter objective lens, Specimen appear light aginst dark background, Increases contrast and enables observation of more details?
    Dark-field Microscopes
  5. Has highest power, Gives credible exciting power?
    Electron Microscope
  6. Increases contrast and resolution by coloring speciemens with stain/dyes?
    Staining
  7. Smears of microorganisms(thin film) air dried to slide and then fixed to surface by heat of chemicals?
    Staining
  8. Microbiological stains are usually salts composed of Cation and Anions and one is colored (chromophore)?
    Staining
  9. Acidic dye stains are alkaline structures; Basic dyes stains acidic structures and are used more commonly?
    staining
  10. Staining: Smears of microorganisms air dried to slide and then fixed to surface by heat of chemicals?
    Thin Film
  11. Staining: Microbiological stains are usually salts composed of Cation and Anions and one is colored?
    Chromophore
  12. Staining: Basic dyes stains acidic structures and are used more commonly? Acidic dye stains?
    Alkaline structures
  13. Types of stains?
    • Simple stains
    • Differential stains
    • Special Stains
  14. Gram stains, Acid-Fast Stains, Endospore stains, are what kind of stains?
    Differential Stains
  15. Negative (capsule) stains, Flagellar Stains, Fluorescent Stains, are what kind of stains?
    Special Stains
  16. Which color has longer wave length violet or red?
    Red
  17. What color is Gram positive?
    Blue
  18. What color is Gram Negative?
    Red

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