Microscopes: LM (Video Microscopy and Image Processing, Super-Res Fluorescence Microscopy)

Card Set Information

Author:
DesLee26
ID:
232329
Filename:
Microscopes: LM (Video Microscopy and Image Processing, Super-Res Fluorescence Microscopy)
Updated:
2013-09-02 09:30:00
Tags:
Cell Bio
Folders:

Description:
Mickle
Show Answers:

Home > Flashcards > Print Preview

The flashcards below were created by user DesLee26 on FreezingBlue Flashcards. What would you like to do?


  1. a.      Video Microscopy and Image Processing
                                                                  i.      some video cameras (like charge-coupled device, or CCD cameras) are made to be __, allowing for imaging at __ (good for __and/ or __ specimen)
                                                                ii.      video cameras can detect and amplify __ within a species so small objects are visible
    • very sensitive to light
    • low illuminations
    • live 
    • fluorescently stained
    • small differences in contrast
  2.                                                               i.      electronic images produced are readily converted to __ with a discrete number of picture elements each of which has an assigned __ and __corresponding to that site in the original image
                                                                ii.      can be stored on pc and edited
    • digital images
    • color and brightness value
  3. Laser Scanning Confocal Microscopy
    - disadvantage of LM
                                                                  i.      different depths of specimen reduce ability of forming a crisp image because those parts interfere with light rays from the plane in focus
  4.                                                               i.      1950: __ invented the __ that produces an image of a __                                                            ii.      specimen is illuminated by a finely focused __ that rapidly scans across the specimen at a __, thus illuminating only a __ within the specimen
    • Marvin Minsky
    • confocal microscope
    • thin plane situated within a much thicker specimen
    • laser beam
    • single depth
    • thin plane
  5.                                                               i.      used with __
    1.      after __at longer wavelength, light from the specimen is brought to focus at a site within the microscope that contains a __ (which, along with the plane are __)
    light rays from the illuminated plane  pass through the aperture, but other rays cannot
    • fluorescent optics
    • reemittence 
    • pinhole aperture
    • confocal
  6. a.      Super-Resolution Fluorescence Microscopy
                                                                  i.      scientists have been able to localize proteins at resolutions in the tens of nm  due to discovery of __
    1.      led to development of __, whose fluorescence emission at a certain wavelength can be switched on and off 
    • certain mutation of GFP polypeptide, which is nonfluorescent until activated by violet light
    • photoswitchable proteins
  7.                                                               i.      STORM (stochastic optical reconstruction microscopy)
    1.      uses __ and allows investigators to localize a single __molecule within a resolution < 20 nm with pulses of light of appropriate wavelengths
    • photoswitchable fluorescent proteins
    • fluorescent
  8. 1.      most labeled molecules remain dark, but some are __ and appear as __

    2.      the center can be determined with high accuracy
    • activated
    • spots hundreds nm wide
  9. 1.      process is repeated for more __, producing a lot of coordinates that represent the locationsof many of the __in the specimen
    2.      analysis leads to  __
    • imaging cycles
    • fluorophores 
    • reconstruction a super-resolution image of the field of view

What would you like to do?

Home > Flashcards > Print Preview