Laboratory Safety

Card Set Information

Author:
MeanEddieG
ID:
232517
Filename:
Laboratory Safety
Updated:
2013-09-02 21:59:51
Tags:
Oertel
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Description:
General Procedures, Working with Bunsen Burners, and Hot Plates, Disposal of Cultures and Other Biohazard Materials, Spills and Accidents
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  1. General Procedures: DONT'S
    Do NOT Eat, Drink, Smoke, or store food in the lab. Avoid all finger to mouth contact.
  2. Genreal Procedures: Lab Coats
    Lab Coats should be worn at all times while working in the laboratory. The Lab coats are to remain in the lab.
  3. General Procedures: Handwashing
    Wash your hands with soap at the beginning and end of the lab period. In addition, wash hands immediately and thoroughly if they become contaminated with microorganisms.
  4. General Procedures: Benches
    Wipe down the lab bench with disinfectant before and after each lab period. Paper towels used only for routine  bench cleaning can be thrown in the regular trash.
  5. General Procedures: Paper Towels
    Any area of paper towels that become stained with dye should be ripped off and disposed of in the appropriate bin, marked hazardous waste.
  6. General Procedures: Books & Bags
    Keep the lab bench clear of any unnecessary books or other items. Store them appropriately.
  7. General Procedure: Googles
    Goggles should be worn whenever using strong acids, bases or other harsh chemicals
  8. General Procedures: Shoes
    Closed toed shoes must be worn in the laboratory at all times
  9. General Procedures: Visitors
    Under NO circumstances are non enrolled visitors allowed in the lab.
  10. Bunsen Burners & Hot Plates: Flammable Items
    Make sure paper towels, text books, alcohol and other lab apparatus are not close to the Bunsen Burner Flames.
  11. Bunsen Burners & Hot Plates: Hair
    Toe back long hair to prevent it from contacting the Bunsen burner flame, hot plates, or from contaminating cultures.
  12. Bunsen Burners & Hot Plates: Smell
    If you smell Gas in the laboratory, check for leaks and notify your instructor immediately!
  13. Disposal of Biohazard: DONT'S
    Do not place anything contaminated with Live Cultures in the regular trash or down the drain.
  14. Disposal of Biohazard: KNOW
    • The Designated areas for Disposal are located on the rear counter of the laboratory
    • a. Biohazard Buckets (Lined with Red Bags) are for the disposal of the Petri Dishes and contaminated paper towels.
    • b. Slide Disposal Bucket (Small Metal Bucket) is for the disposal of student prepared slides and other temporary slides.
    • c. Used Culture Tubes are placed in the designated racks. Sort Tubes according to their size, type of closure (metal, screw cap, or foam plug) and tube type.
    • d. Used Pipettes and Disposable Swabs are placed tip down in a pipette disposal jar.
  15. Disposal of Biohazard: SINK
    Stains containing  PHENOL  and Rinse Residue MUST go into the haz-mat disposal container, NEVER DOWN A SINK. DO NOT fill waste Haz-Mat waste bottles to rim.
  16. Spills & Accidents: Instructor
    Notify the Instructor of any spill, breakage, or other accident.
  17. Spills & Accidents: SPILLS - MICROORGANISMS
    For Spills of liquids containing microorganisms on the bench top or floor, saturate the spill with disinfectant solution and cover the area with paper towels for 10 minutes. Place the contaminated paper towels iin the biohazard disposal buckets. Ask the instructor for help.
  18. Spills & Accidents: SPILLS - PAPERS
    For spills on papers or Lab Notebooks, blot the spill with a paper towel and place the paper towel in the biohazard bucket. Place the contaminated notebook or paper in the ultraviolet cabinet and turn the UV light on for 15 minutes.

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