bio 115

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bio 115
2013-09-05 20:31:34
lab Ex microscopy pg

lab notes
Show Answers:

  1. what is the name of the microscope
    Bright field light microscope
  2. what does the bight field light microscope do
    produces dark image against light background
  3. who is the bright field light microscope most comminly used by
    biology students
  4. what is the bright field light microscope used for
    • to view preserved stains
    • which means dead specimen with dye on it
  5. what is the framework of the microscope
    • arm
    • base
    • head which rotates
  6. what is the stage
    fixed platform that supports the microscope slide
  7. what is the mechanical stage
    • allows horizontial and vertical movement
    • allows to center the specimen
  8. what is the light source
    • its build into base
    • round knob allows you to control intensity of light
  9. what is the focusing knob
    concentrically arranged knobs on side of microscope that brings specimen into focus
  10. what are the focusing knobs on the microscope
    • coarse adjustment
    • fine adjustment
  11. what is the coarse adjustment
    • larger
    • outer knob
    • rough image
  12. what is the fine adjustment
    • smaller
    • inner knob
    • fine image
  13. what are the 3 types of lenses
    • ocular lens
    • objective lens
    • condenser
  14. ocular lens
    • eye piece
    • binocular because it has 2
    • magnification of 10x
  15. objective lens
    the 4 lens that are attached to rotating nosepiece that allows them to move over a slide
  16. what are the 4 objective lens
    • 4x
    • 10x
    • 40x
    • 100x
  17. 4x
    • scanning
    • shortest
    • red
  18. 10x
    • low power
    • yellow
  19. 40x
    • high dry
    • blue
  20. 100x
    • oil immersion
    • white
    • need to use drop of oil
    • lenses need to touch the oil for it to work
  21. condenser
    • below stage
    • lens that collect and direct the light from the lamb to  the slide being studied
  22. what does the condenser do that the ocular and obective lens doesn't
    does not effect the magnifying power
  23. what is the part within the condenser and what does it do
    • diaphragm which controls amount of light that reaches the slide
    • also known as iris diaphragm
  24. what are the 2 jobs of the microscope
    • magnification
    • resolution
  25. what do we mean when we say one of the microscopes job is magnification
    ability to enlarge objects
  26. what do we mean when we say one of the microscopes job is resolution
    capacity to distinguish or separate two adjacent objects as separate
  27. what are the two phases that magnification occurs in
    the objestive lens magnifies and then the ocular magnifies again to form an image
  28. what is the formula to calculate total magnification
    power of objective X power of ocular= total magnification
  29. what is the purpose of oil
    • to keep the light into image
    • prevents refraction of light
  30. what is the total magnification of each objective lens
    • 4x=40x
    • 10x=100x
    • 40x=400x
    • 100x=1000x
  31. what are the steps to use the microscope
    • center specimen
    • start with low power objective (10x)
    • turn coarse adjustment knob until image appears
    • bring object into focus with fine adustment
    • once image is visible progess to next objective
  32. what do you never want to do after focusing the image with fine adjustment
    do not readjust coarse objective knob
  33. what sould you do before moving objective to oil immersion
    • place a drop of oil on the slide
    • go half way between objectives, put the drop if oil, then finish sliding objective into place
    • should be touching oil
  34. transport
    • always carry with 2 hands
    • one hand on base
    • other hand on arm
    • do not let cord dangle to aviod tripping
  35.  what sould you clean lenses with
    • lens paper and solution if needed before and after use
    • clean oil immersion last
  36. cleaning the microscope
    • remove the slide
    • clean lenses
    • lower stage
    • rotate to scanning objective because less damange
    • before putting microscope away have teacher check it
    • wrap cord
    • replace dust cover
    • put under proper location