BCHM 307

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Author:
Anonymous
ID:
233294
Filename:
BCHM 307
Updated:
2013-09-06 20:24:03
Tags:
Protiens
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Description:
TA's lectures
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  1. 2-D gel electrophoresis
    • 1. by isoelectric point (pH)
    • 2. by size
  2. Proteases
    • enzymes that cleave the peptide bond
    • site-specific ones used for ID protiens
    • e.g. trysin after lys, arg
    • Some chemicals can be used also CNBr
  3. In-gel Protease Digestion/ Peptide Mass Fingerprinting (PMF)
    • Run gel with whole protien
    • Cut out section
    • resolve in Protease solution
    • Mass Spectrometer = graph
    • anaylize where breaks
  4. Requirements for PMF
    • Genome of organism sequenced
    • need site-specific protease
    • need high accuracy mass spectrometer

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