BioChemTest1Ch3

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Author:
victimsofadown
ID:
234121
Filename:
BioChemTest1Ch3
Updated:
2013-09-15 03:29:17
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BioChemTest1Ch3
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BioChemTest1Ch3
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  1. Information about AA titration curves
    • # stages = # deprotonations
    • low pH = fully protonated
    • at midpoint [HA]=[A-] and pH = pKa *known value
    • curve implies multiple point of buffer power
    • pI (isoelectric point): pH when net charge = 0 (halfway between +1,0 pka values)
  2. Process for performing a peptide titration curves
    • AA are joined by amide bonds
    • Look to see if any AA have ionizable side chain (pKr)
    • Assuming a 3 AA peptide.... (I, II, III)
    • 1) determine the equivalent OH- portions in NaOH
    • 2) plot the known values
    • 0.5 Eq OH- = pKa1
    • 1.5 Eq OH- = pKr
    • 2.5 Eq OH- = pKa2
    • 3) determine the endpoints by averaging the known pKa values for 1 Eq OH- and 2 Eq OH-
    • 4) determine the initial pH by using a RICE table with the pKa converted to Ka
    • 5) determine the final pH by using
  3. Separate the amino acids by side chain characteristics
    • Nonpolar, aliphatic: glycine, alanine, proline, valine, leucine, isoleucine, methionine
    • Aromatic: Phenylalanine, tyrosine, tryptophan
    • Polar, uncharged: serine, threonine, cysteine, asparagine, glutamine
    • Positively charged: Lysine, histidine, arginine
    • Negatively charged: aspartate, glutamate

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