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  1. Parts of a microscope
    • Ocular Lens
    • Objective Lens
    • Stage
    • Light Source
    • Base
  2. What is a compound microscope?
    An objective and ocular lens is used to compound magnification
  3. Steps of a gram stain
    • Apply crystal violet dye to slide
    • Apply iodine to slide to make dye more stay-fast
    • Decolorize with alcohol to remove dye from gram negative bacteria
    • Apply safranin to slide
  4. What color does Gram positive/negative bacteria stain?
    • Positive - Blue/purple
    • Negative - Red/pink
  5. Steps to extract DNA:
    • Collect Cells
    • Lyse cells to collect DNA
    • Separate DNA from protien and debris
    • Isolate concentrated DNA
  6. What are histones, detergent and protinease K?
    • Histones - bundles that DNA are wrapped around inside the nucleus
    • Protinease K - element in lysis solution that unravels histones
    • Detergent - Breaks cell wall/membrane so DNA can be released
  7. What does the salt do while isolating DNA?
    Causes protien and other debris to clump together so DNA can be easily seen
  8. Gram positive vs. Gram negative bacteria
    • Differences in cell walls:
    • Gram positive has 20% more PTG
  9. 6 common gram positive bacteria that infect humans:
    • Streptococcus (cocci)
    • Staphylococcus (cocci)
    • Bacillus(bacilli)
    • Clostridium (bacilli)
    • Cornyebacterium (bacilli)
    • Lysteria (bacilli)
  10. Examples of Gram Negative bacteria:
    • Spirochetes - (spiral-shaped)
    • Neisseria (cocci)
  11. What is a mycoplasma?
    • A bacteria that has no cell wall.
    • Penicillins aren't effective
    • Bacteria change shape, or are "Pleomorphic"
    • Smallest bacteria capable of self-reproduction
  12. How does transcription work?
    • A - U
    • T - A
    • C - G
    • G - C
  13. What is a Type I Hypersenstitivity?
    • IgE mediated
    • Antigen causes crosslinking of IgE antibodies bound to IgE receptors of mast cells.
  14. What is a Type IV Hypersensitivity?
    • Delayed hypersensitivity
    • T-Cell mediated
    • T-Cells release cytokines
  15. TB - What kind of mask?
  16. Meningitis - What kind of mask?
    Surgical Mask
  17. Flu - What kind of mask?
    Surgical Mask
  18. Chickenpox - What kind of mask?
    No mask.
  19. What goes in a sharps box?
    Anything sharp
  20. What goes in a trash can?
    • Trash
    • Gloves
    • Used bandaids
    • Gauze with DRY substances
  21. What goes in a biohazard container?
    Anything with WET fluids/substances on it.
  22. Patient with Encephalitis - precautions:
    Standard Precautions
  23. Scabies - What kind of precautions?
    Contact Precautions
  24. Meningococcal Meningitis - What type of precautions?
    Respiratory precautions
  25. SARS - What kind of precautions?
    • Respiratory
    • N95
    • Contact
  26. VRE - What kind of precautions?
    Contact precautions
  27. Tuberulosis - What kind of precautions?
    • Respiratory
    • N95
  28. Anthrax - What kind of precautions?
    Standard precautions
  29. Hep A, B, C - What type of precautions?
    Standard precautions
  30. What types of precautions are needed for the following situations?
    Managing trauma patient
    Changing IV dressing
    Cleaning Incontinent patinet
    Taking blood pressure
    Irrigating large open wound
    Inserting catherter
    • Managing trauma patient - Handwashing, Gloves, Gown, Faceshield
    • Changing IV Dressing - Handwashing, Gloves
    • Cleaning Incontinent Patient - Handwashing, Gloves, Gown
    • Taking Blood Pressure - Handwashing
    • Irrigating Large Open Wound - Handwashing, Gloves, Gown, Faceshield
    • Inserting Catherter - Handwashing, Gloves

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2010-06-14 22:46:14

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