Phenol red broth

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justjay18xx
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235501
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Phenol red broth
Updated:
2013-09-17 23:44:49
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Phenol red broth
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Phenol red broth
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  1. a lactose fermenter is an organism that splits the _______ lactose into ______and _____ then ferments the _________
    • 1.dissacharides 
    • 2.monosaccharides 
    • 3.glactose
    • 4.then ferments the monosaccharides
  2. phenol red broth contains a fermentable ____. Included in the base medium are _____ and the pH indicator ______
    • 1.carbohydrate 
    • 2.peptone 
    • 3.phenol red
  3. phenol red is _____ below pH 6.8 and ____ above pH 7.4
    • 1. yellow 
    • 2.magenta
  4. as prepared the phenol red broth is at pH_____and appears red
    1. 7.3
  5. ___ peptone amino acids produces ____ which raises the ph and turns the broth ____
    • 1. deamination 
    • 2. ammonia Nh3 
    • 3. pink
  6. an inverted durham tube is placed inside the tube to
    to detect gas production
  7. if the medium is inoculated, incubated and then observed to produce a pink result this indicates an alkaline condition caused by deamination. So reading carbohydrate fermentation test can be unreliable if "reversion has occurred. Why?
    fermentation test would have a unrliable test result due to "reversion" because when reversion occurs there is a phenomenon of a microorganism fermentively depleting the carbohydrate and reverting to amino acid metabolism, thereby neutralizing acid products with alkaline products; produces a false negative. So a
  8. define reversion
    In carbohydrate fermentation tests, the phenomenon of a microorganism fermentively depleting the carbohydrate and reverting to amino acid metabolism, thereby neutralizing acid products with alkaline products; produces a false negative.
  9. how many carbohydrates are added to phenol red broth and why?
    contains any one of several carbohydrates (e.g., glucose, lactose, sucrose) and a pH indicator to detect acid formation. MR-VP broth is a dual-purpose medium that tests an organism’s ability to follow either (or both) of two specific fermentation pathways.
  10. mixed acid  fermen tation.
    identifies bacteria that are able to produce acetoin as part of a 2,3-butanediol fermentation.
  11. what is PR base broth
    PR broth is used to differentiate members of Entero­bacteriaceae and to distinguish them from other Gram-negative rods.
  12. Phenol Red Broth is a medium that contains a protein called
    peptone

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