The flashcards below were created by user
justjay18xx
on FreezingBlue Flashcards.
-
list the gram reaction and morphology of th enteric bacteria
Gram+poor growth or no growth, organism is inhibited by crystal violet and/or bile
Gram-Good growth, organism is not inhibited by crystal violet or bile
-
The selective agent for MacConkey Agar
bile salts and crystal violet and Ferric ammonium citrate
-
indicators for MacConkey Agar
neutral red
-
Basis for differentation for MacConkey Agar
lactose
-
Basis for differentation for MacConkey Agar
lactose
-
MacConkey Agar inhibits the growth of Gram ____ bacteria
positive
-
MacConkey Agar supports the growth of Gram ____ bacteria
negative
-
Probable coliform
pink to red growth with or w/o bile precipitate, organism produces acid from lactose fermentation
-
Noncoliform
growth is "colorless" (not red or pink), organism does not ferment lactose. No reaction
-
MacConkey Agar has ____ numer of sugars
one
-
Selective media
suppresses growth of unwanted bacteria and encourages growth of desired microbes.
-
Differential media
facilitates distinguishing the desired microbe from others in a mixed sample
-
Enterobacteriaceae
family name; includes gram (-) organisms found in human GI tract.
-
Enterics
general term for the enterobacteriaceae family members.
-
Fecal Coliforms
are a commonly used bacterial indicator of sanitary quality of foods and water
-
Lactose
a disaccharide composed of glucose + galactose
-
Enteric lactose fermenters (LF) such as E. coli , Enterobacter, and Klebsiella are Less pathogenic or more pathogenic?
They are less pathogenic ( Non pathogenic)
-
Non-Lactose Fermenters like Salmonella, Shigella, Proteus are Less pathogenic or more pathogenic?
They are more pathogenic
-
Mention the organisms that are Lactose Fermenters
E. coli, Enterobacter, Klebsiella pneumoniae
-
Mention the organisms that are Non-Lactose fermenters.
Salmonella, Shigella, Proteus, Morganella morganii
-
Mention the three types of agar used to identify gram (-) microbes
MacConkey AgarEosin Methylene Blue agar (EMB)Hektoen Enteric agar (HE/SS)
-
MacConkey agar is
both selective and differential
-
MacConkey agar "selects" for ..
- Gram (-)- suppresses growth of gram (+)- Crystal violet and bile salts inhibit growth of gram (+) due to inhibition of cell wallpeptidoglycan synthesis.
-
In MacConkey agar " Crystal violet and bile salts" inhibit the growth of gram (+) due to
Crystal violet and bile salts" inhibit the growth of gram (+) due to ....inhibition of cell wall peptidoglycan synthesis.
-
MacConkey agar "differentiates" between
lactose fermenters and non-lactose fermenters- Lactose provides the sugar substrate for fermentation- Neutral red dye is a pH indicator which turns red when acidic and is colorless when basic.
-
In MacConkey agar "end products of lactose fermentation increase acidity of media and turns pH indicator what color?
Red
-
Results of MacConkey agar
- Gram (+) will not grow on this media- Lactose fermenters grow pink/red-Non-lactose fermenters grow colorless colonies
|
|