lysine iron agar
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how is lysine iron agar differential
LIA is used to differentiate enterics based on their abilityto decarboxylate or deaminate lysine and produce hydro- gen sulfide (H 2 S).
the fermentable carbohydrate in lysine iron agar is
the primary substrate for deamination and decarboxylation reactions in this experiment is
the pH indicator in this medium is broncresol which is _____ at pH 6.8 and higher and___ at ph 5.2 and below
thiosulfate when reduced becomes
ferric iron ion in this experiment reacts with hydrogen sulfide to for
this medium proves an ____ zone for growth on the slant and an ____ zone for growth in the butt
- 1. oxygen aerobic
- 2. no oxygen anaerobic
what are all typical results and interpretations in this experiment
Medium is darkened within 48 hours Presumptive identification as a member of Group D Streptococcus or Enterococcus1
No darkening of the medium after 48 hours Presumptive determination as not a member of Group D Streptococcus or Enterococcus
decarboxylation compare how he conditions oxygen levels are created with this experiment versus the moellers decarboxylase broth with lysine
what is the selective agent
; bile is the selective agent added to separate the Streptococcus bovis group and e nterococci from other streptococci.
demonstrate tolerance to bile
Lactococcus lactis Enterococcus faecalis surrogate (Serratia marcescens)
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