ClinLab4: Mycology (Molds and Media)

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Author:
heather.dundas
ID:
236781
Filename:
ClinLab4: Mycology (Molds and Media)
Updated:
2013-09-24 15:14:38
Tags:
vti
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vti
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  1. What are two forms of mold?
    • saprophytes
    • dermatophytes
  2. Which mold is free living?
    saprophyte
  3. Where are saprophytes found?
    breads and cheeses
  4. Which mold causes skin infx?
    dermatophytes
  5. Molds are fungi that grow with _ hyphae.
    aerial
  6. Molds appear _ on media.
    fuzzy/fluffy/furry
  7. Molds grow by branching and produce _ bodies.
    fruiting
  8. The fruiting body is the main reproductive part of mold. What does it produce?
    spores
  9. Life cycle of mold:HG,FB/SF,S,SG
    • hyphal growth
    • fruiting body/spore formation
    • spores
    • spore germation
  10. Name 2 media for molds:
    • DTM
    • Derm-duet
  11. What is derm-duet selcective for?MTE
    • microsporum
    • trichophyton
    • epidermophyton
  12. What does microsporum cause?TTRO
    • tinea capitis
    • tinea corpus
    • ring worm
    • other dermatophytosis
  13. What does trichophyton cause?
    dermatophytosis in hair, skin, and nails of humans
  14. What does epidermophyton cause?
    superficial and cutaneous mycoses
  15. What is the most common mold media?
    DTM
  16. DTM aka
    fungassay
  17. What does DTM contain?
    abx to inhibit growth of bacteria and most other media
  18. What is DTM selective for? MT
    • microsporum
    • trichophyton
  19. With DTM how long does it take for a change in color?
    72 hrs
  20. After DTM is labeled, inoculated, and incubated at room temp where is it stored?
    somewhere dark
  21. Should the cap ever be tight on DTM?
    no keep it loose
  22. What does a (+) dermatophyte (+) ringworm DTM look like?
    • white-apricot fluffy growth
    • media turns red
  23. What does a (+) saprophyte (-) ringworm DTM look like?
    • black/brown/dark green growth
    • no color change of media
  24. What are the two halves of a derm-duet?
    • DTM
    • RSM
  25. What does RSM stand for?
    rapid sporulation media
  26. What is RSM selective for?ME
    • microsporum
    • epidermophyton
  27. How long is a derm-duet incubated at room temp?
    10hrs max
  28. In a derm-duet how long does it take to get a (+) trichophyton red media color change?
    3d
  29. How long does it take to get a (+) RSM? What color?
    3-7d blue/green
  30. Purpose of derm-duet
    detection and specification of dermatophytes

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