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2013-09-26 20:50:05
Connective Tissue Part Cont

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    • Irregular dense connective tissue human scalp – X64.
    • collagen is brown, and blue is nuclei of fibroblasts. We know its irregular because the bands of collagen fibers are running in all different directions.
    • Irregular dense connective tissue – human scalp  X256
    • Dark spots—fibroblasts
    • Loose (around the glandular tissue, red arrow) and irregular dense (upper and lower right parts of the micrograph) connective tissue from inactive mammary gland.  Masson trichrome.  X 200. 
    • Human breast glandular epithelium.
    • Irregular dense connective tissue from human dermis contains thick bundles of collagen fibers, fibroblast nuclei (arrowheads), and a few small blood vessels (bv).
    • H&E stain. Medium magnification.
  1. Irregular dense connective tissue capsule of testis. X=aggregated collagen fibrils; X 9.500.  Inset: high mag of collagen fibrils. Arrows indicate periodicity.  75,000X.
  2. Reticular tissue in the spleen consists of meshwork of reticular fibers.  The spleen is covered by dense connective tissue capsule (right) from which a trabecula is extended into the stroma of the spleen
  3. Cortex of a lymph node contains reticular tissue.  Wilder stain.
    • Reticular fibres form a delicate supporting framework for many highly cellular organs such as lymph nodes and liver (this picture).
    • Red—nuclei, cytoplasm not visible, reticular fibers support the parenchyma
    • A-single muscle cell
    • B-two muscle cells in diff. orientaReticular tissue around skeletal muscle of the tongue.
    • Muscle cells are surrounded by basal lamina which is external lamina made of reticular fibers
    • Red arrow-cut at level of reticular fibers, we see the reticular fibers covering cell from the outside
  4. Reticulum network, skeletal muscle, tongue.
  5. Reticulum, kidney tubule—convoluted tubules, and each tubule made of simple cuboidal cells resting on basal lamina with reticular fibers on the outside of the basal lamina
  6. Reticulum;  gall bladder, simple columnar epithelium cells, then the reticular fibers red arrows
  7. Reticulum, ducts and acini, salivary gland, monkey
    • Dense regular elastic connective tissue.  The white spaces among the elastic fibers represent unstained loose connective tissue elements and parallel rows of flattened fibroblast that can not be distinguished in this preparation.  LONGITUDINAL SECTION
    • Tunica media of aorta made of laminae
    • Dense regular elastic tissue in cross section.  
    • Middle of the upper part of the micrograph represents a blood vessel surrounded by unstained loose connective tissue elements that can be seen, as well, as white areas among the elastic fibers. TRANSVERSE SECTION
  8. Elastic fiber.  X 40,000.  E=elastin; arrows, fibrillin microfibrils; C=collagen
    • Elastic tissue layers (sheets)  human aorta.
    • Tunica Media
    • Brown—laminae of elastic tissue
    • Elastic fibers made by smooth muscle, only in tunica media
  9. TEM of human collagen fibrils in cross and longitudinal sections. Each fibril consists of regular alternating dark and light bands that are further divided by cross-striations. Ground substance completely surrounds the fibrils. x100,000.
  10. Collagen Fiber Assembly diagram
  11. collagen fiber
    • Electron micrograph of cross sections of reticular (left) and collagen (right) fibers.
    • Note that each fiber type is composed of numerous smaller collagen fibrils.
    • Reticular fibrils (R) are significantly narrower in diameter than collagen fibrils of collagen fibers (C; see histogram inset).  In addition, the constituent fibrils of the reticular fibers reveal an abundant surface-associated granularity not present on regular collagen fibrils (right). x70,000.