HISTO BLOCK II

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Author:
nikistrow
ID:
237403
Filename:
HISTO BLOCK II
Updated:
2013-09-27 17:37:08
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Connective Tissue Part
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    • Mast cells releasing histamine
    • Blebbing of outside of cell is granules
    • being released.
  1. Mast cells, neutral red
  2. Mast cells and macrophages, lithium-carmine injection; toluidine blue
    • A-nucleus of mast cell
    • B-cytoplasmic granules of mast cell
  3. Mast cells near blood vessels (red arrows), skin rat, neutral red. Whole mount slide
    • A-muscle cell
    • B-5 mast cells
    • C- blood vessel
    • LM image of Kupffer Cells (black cells), sinsoids are white areas 
    • Kupffer are liver macrophages
  4. Macrophage-langhan's cell
    • Langhans’ Cell. Note the horseshoe arrangement of the nuclei.
    • Red arrow, fused nuclei
    • A-macrophage
    • B-eosinophil
    • Electron micrograph of several macrophages and 2 eosinophils in a region adjacent to a tumor. This figure illustrates the participation of macrophages in tissue reaction to tumor invasion.
  5. macrophage in spleen of dog
    • A- RBC
    • B-fenestrated sinusoid
    • C-arm of macrophage
    • SEM of a macrophage cytoplasmic process in rat liver.  A macrophage (kupffer’s cell) extends a cytoplasmic process in recognition of a damaged (previously fixed in glutaraldehyde for a few minutes) erythrocytes in the initial stage of phagocytosis.
    • A-lymphocyte
    • B-macrophage
    • Red arrow- contact between lymphocyte and macrophage
    • Electron micrograph of a macrophage. Note the lysosomes (L), the nucleus (N), and the nucleolus (Nu). The arrows indicate phagocytic vacuoles.
    • Phagocytosed material is destroyed by the
    • action of lysosomes.  
    • The end product of digestion is either recycled or exocytosed. 
    • Inert particles like inhaled carbon will resist digestion and may remain in the cytoplasm indefinitely as residual bodies
    • P=phagolysosome, L=lysosome
    • A-early endosome
    • B-lysosomes
    • TEM of macrophage exhibiting endocytotic vesicles, early and late endosomes, lysosomes, and phagolysosomes. X 10,000.
  6. When macrophages take up massive amounts of cholesterol they form “foam cells,” characterized by multiple lipid droplets (stained red).
  7. Section of pancreas from a rat injected with the vital dye trypan blue. Note that 3 macrophages (arrows) have engulfed and accumulated the dye in the form of granules. H&E stain. Low magnification. Darker spots are lysosomes found within the macrophages
    • Arrows to bundles of actin in cytoplasm.
    • Fatter arrows to collagen fibrils
    • A-Fibroblast
    • B-Collagen
    • A-RER
    • B-Golgi
  8. connective tissue layer (dermis) shows several fibroblasts (F), which are the elongated cells. H&E stain. Medium magnification.
  9. Quiescent fibroblasts are elongated cells with thin cytoplasmic extensions and condensed chromatin. Pararosaniline-toluidine blue (PT) stain. Medium magnification.
    • A- fibroblast
    • B-Collagen
    • C-cytoplasmic extension
  10. fibroblasts

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