Mast cells and macrophages, lithium-carmine injection; toluidine blue
A-nucleus of mast cell
B-cytoplasmic granules of mast cell
Mast cells near blood vessels (red arrows), skin rat, neutral red. Whole mount slide
B-5 mast cells
C- blood vessel
LM image of Kupffer Cells (black cells), sinsoids are white areas
Kupffer are liver macrophages
Langhans’ Cell. Note the horseshoe arrangement of the nuclei.
Red arrow, fused nuclei
Electron micrograph of several macrophages and 2 eosinophils in a region adjacent to a tumor. This figure illustrates the participation of macrophages in tissue reaction to tumor invasion.
macrophage in spleen of dog
C-arm of macrophage
SEM of a macrophage cytoplasmic process in rat liver. A macrophage (kupffer’s cell) extends a cytoplasmic process in recognition of a damaged (previously fixed in glutaraldehyde for a few minutes) erythrocytes in the initial stage of phagocytosis.
Red arrow- contact between lymphocyte and macrophage
Electron micrograph of a macrophage. Note the lysosomes (L), the nucleus (N), and the nucleolus (Nu). The arrows indicate phagocytic vacuoles.
Phagocytosed material is destroyed by the
action of lysosomes.
The end product of digestion is either recycled or exocytosed.
Inert particles like inhaled carbon will resist digestion and may remain in the cytoplasm indefinitely as residual bodies
TEM of macrophage exhibiting endocytotic vesicles, early and late endosomes, lysosomes, and phagolysosomes. X 10,000.
When macrophages take up massive amounts of cholesterol they form “foam cells,” characterized by multiple lipid droplets (stained red).
Section of pancreas from a rat injected with the vital dye trypan blue. Note that 3 macrophages (arrows) have engulfed and accumulated the dye in the form of granules. H&E stain. Low magnification. Darker spots are lysosomes found within the macrophages
Arrows to bundles of actin in cytoplasm.
Fatter arrows to collagen fibrils
connective tissue layer (dermis) shows several fibroblasts (F), which are the elongated cells. H&E stain. Medium magnification.
Quiescent fibroblasts are elongated cells with thin cytoplasmic extensions and condensed chromatin. Pararosaniline-toluidine blue (PT) stain. Medium magnification.