Connective Tissue Lecture 2 part I

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Connective Tissue Lecture 2 part I
2013-09-28 10:16:24
saba sabamed Connective Tissue Lecture part

Connective Tissue Lecture 2 part I
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  1. What kind of fibers are these? What type of collagen are they usually made of? Name 2 places you can find them.
    Reticular fibers. Type III. Reticular fibers form a delicate supporting framework for highly cellular organs such as lymph nodes and liver (this picture).
  2. What kind of fibers are these? What 2 stains are used and why?
    nEasily demonstrated with the PAS reaction•Due to higher sugar content than regular collagen due to glycoproteinsneasily demonstrated with silver staining procedure•i.e. they are argyrophilic – affinity for silver stain
  3. What kind of fibers are these? Why are they useful? How can you tell apart from collagen fibers?
    Enable tissues to stretch and distend. Interwoven with collagen to prevent tearing. Thinner than collagen Branching  pattern•form 3-D network. Fibers usually appear homogeneous compared to collagen.  large amounts give a yellowish color to tissues.
  4. Elastic fiber.  X 40,000.  A=elastin; arrows, fibrillin microfibrils; B=collagen
  5. TEM of elastic fiber from ligamentum nuchae. Note that the fibrillin microfibrils (B/F) surround the amorphus elastin (A/E).  (a) and (b) from 3-month fetal calf.  (c) and (d) from 9-month fetal calf (at term). C: ElastinEarly elastogenesis we see only fibrils in microfibrils (left top), and only see fibril sat the periphery once elastins and microfibrils are formed.
  6. What kind of tissue is this and where is this from? Distinguishing characteristic? Where are elastic fibers made from?
    Elastic tissue from Elastic artery; aorta – darker lines are lamalle. Elastic fibers from smooth muscle cells in ELASTIC ARTERIES, NOT from fibroblast.
  7. Immunohistochemistry of section obtained from the tongue shows that laminin (orange) is restricted to the basal lamina of the epithelium C: (E, top of the picture) and to the muscle fibers D: (M), B: nerves (N), and B: small blood vessels (lower half of picture).  Each circle is a cell and surrounded. Schwann cells surrounded by basal lamina. External lamina.
  8. Label A & B
    A: Proteoglycans; vertical rod core of protein molecules of glycosaminoglycans (GAGs) are covalently bound unbranched polysaccharide (repeating disaccharides amino sugar + uronic acid. Proteoglycans contains MORE of carbohydrate than glycoproteins.

    B: Glycoproteins are globular protein molecules to which branched chains of monosaccharides are covalently attached.
  9. Ground substance GAG (glycosaminoglycans) on proteoglycans include what 5 sugars and acid?
    • Christmas does kill: ho-ho-ho
    • C- Chondroitin Sulfate
    • D- Dermatan Sulfate
    • K- Keratan Sulfate
    • H- Heparin
    • H- Heparan Sulfate
    • H- Hyaluronic Acid
  10. What Extra Cellular Matrix protein is this? What is it responsible for?
    • AGGRECAN - main ECM proteoglycan in cartilage: Chondroitin sulfate + keratan sulfate
    • •Interacts
    • with type II collagen
    • Makes a compression-resistant gel
    • Joint problems with age because proteoglycan looses its capacity