HISTO BLOCK II CARTILAGE

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Author:
nikistrow
ID:
237908
Filename:
HISTO BLOCK II CARTILAGE
Updated:
2013-09-30 14:54:10
Tags:
Cartilage Part
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Description:
Saba
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    • 1. Capsular or pericellular matrix (arrow)
    • -Highest conc of sulfated proteoglycans
    • - Found in immediate vicinity of chondrocytes
    • -Stains intensively with basic dyes

    • 2- Territorial matrix (circled around the cells)
    • -Surrounds the isogenous group  of chondrocytes
    • -Stains less intensively
    • -Lower conc of sulfate proteoglycans

    • Interterritorial matrix (odd shaped circle)
    • - Occupies the spaces between groups
    • of chondrocytes.  Weak basophilia
    • - Lowest conc of proteoglycans
  1. Metachromasia showing capsular, territorial, and interterritorial maxtrix areas
  2. ACTIVE chondrocytes show areas of basophilic cytoplasm (RER) and prominent Golgi (arrows)
  3. Chondrocytes. Clear areas represent areas of glycogen and lipids
    • Active chondrocyte
    • A-rER
    • B-Golgi
    • C-Nucleus
    • D-Mitochondria
  4. Trachea is lined by a psuedostratified ciliated columnar epithelium (EP).  Chondrocytes (C) are visible within the mass of hyaline cartilage that make the tracheal ring.  The cartilage is covered from both sides by pericondrium (P).  V, blood vessel
    • A-Perichondrium
    • B-Chrondoblast
    • A-perichondrium
    • B-Chondrocyte
    • C-chondroblast
    • Hyaline cartilage
    • A-pseudostratified ciliated columnar epithelium
    • B-perichondrium
    • C-chondrocytes
  5. Perichondrium (arrows) surrounds a growing hyaline cartilage.
  6. hyaline cartilage, surrounded by perichondrium
  7. Hyaline cartilage is surrounded by perichondrium (Peri) which consists of two layers; outer fibrous layer (F) composed of mostly collagenous fibers and fibroblasts, and an inner more cellular chondrogenic layer (CG) that gives rise to chondroblasts (CB).  As newly differentiated chondroblast starts to secrete ECM they eventually became surrounded by their secretion and called condrocytes (CC).
  8. hyaline cartilage incorporating fibers. silver stain
  9. The surface of the articular cartilage (solid arrows) and the interface between the cartilage and bone (dashed arrows) are free of perichondrium.
  10. elastic cartilage, resorcin stain
  11. Elastic cartilage in middle of epiglottis
  12. ELASTIC CARTILAGE. KEY: AT, adipose tissue; E, elastic fiber; EC, elastic cartilage; MG, mucous gland; P, perichondrium; SE, stratified squamous epithelium. Epiglottis human.  H&E plus orcein 1= X80;  2= X 250
  13. Higher magnification of the elastic cartilage in the epiglottis.  P, perichondrium; CC, chondrocyte; F, elastic fibers.
  14. elastic cartilage
  15. epiglottis, elastic cartilage with perichondrium
  16. Epiglottis.  Elastic cartilage.  Note the elastic fibers (F) among the condrocytes (C).  Adipocytes (A) appear at the left side (upper and lower corners).

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