Microbio lab 3 (streaking, types of media, spread-plate technique, etc.)

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mikepl103
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238558
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Microbio lab 3 (streaking, types of media, spread-plate technique, etc.)
Updated:
2013-10-03 13:36:23
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Microbio lab streaking types media spread plate technique etc
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Microbio lab 3 (streaking, types of media, spread-plate technique, etc.)
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  1. what are the three types of media?
    • 1. liquid/broth
    • 2. semisolid (contains agar solidifying agent)
    • 3. solid (contains agar solidifying agent)
  2. what is the liquid/broth medium used for?
    to propogate a large # of microorganisms
  3. what is semisolid medium used for
    • to determine bacterial
    • motility and promote anaerobic growth
  4. what is solid medium used for?
    • observe surface
    • growth of microorganisms & pure culture isolations also for storage of
    • cultures.
  5. if liquid media poured into test tube and solidified, can become what?
    Test tube for agar slants and agar deep tube.
  6. if liquid media poured into plate and solidified, it becomes
    agar plate
  7. if liquid media solidified in broth tube, it becomes
    turbid (cloudy) solution if bacteria are still present.
  8. chemically defined/synthetic media are
    • consist of known amounts
    • of pure chemicals.  Such media are used
    • in culturing autotrophic microorganisms like algae or non Fastidious heterotrophs
  9. complex/non-synthetic media
    • made of materials rich in vitamins
    • & nutrients usually beef extract, yeast extract & peptones.

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