Practical 1 - Microbiology 251

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lilSunshine08
ID:
238778
Filename:
Practical 1 - Microbiology 251
Updated:
2013-10-05 02:59:55
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MIcro
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Micro
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  1. BSL #1
    (Biosafety Levels)
    Health Risk/practices/Primary barries/ 2ndary Barries/organisms
    • 1. Not know to cause any disease in healthy humans
    • 2.Standard open bench microbiology
    • 3.None required
    • 4.open bench-sink, googles and gloves for liquids
    • 5.Many used
  2. BSL #2
    (Biosafety Levels)
    Health Risk/practices/Primary barries/ 2ndary Barries/organisms
    • 1.Can cause disease in healthy H. easily contained.
    • 2.BSL 1 + bio-hazard warning signs
    • 3. Class II bio-safety cabinet, googles, mask, lab coats, gloves.
    • 4.BSL #1 plus autoclave
    • 5. Some used
  3. BSL #3
    (Biosafety Levels)
    Health Risk/practices/Primary barries/ 2ndary Barries/organisms
    • 1.May have serious or lethal consequenses
    • 2.#2 plus controlled access
    • 3.BSL2 plus BSC use for all procedures
    • 4.BSL2
    • 5.None used
  4. BSL #4
    (Biosafety Levels)
    Health Risk/practices/Primary barries/ 2ndary Barries/organisms
    • 1.High risk of life threatening
    • 2.BSL3
    • 3.BSL3 plus complete isolation
    • 4.BSL3
    • 5.None used
  5. What are the total Magnification of the microscope?
    • 4x * 15 = 60x
    • 10x * 15 = 150x
    • 40x * 15 = 600x
    • 100x * 15 = 1500x
  6. 13.
    http://home.roadrunner.com/~lubehawk/BioHELP!/scopefun.jpg
    Oculator Lens

    Lens located in eye piece with 15x magnification
  7. 7.
    http://home.roadrunner.com/~lubehawk/BioHELP!/scopefun.jpg
    Objectives

    • lenses on revolving nose piece
    • 4x, 10x, 40x, and 100x magnification
  8. 6.
    http://home.roadrunner.com/~lubehawk/BioHELP!/scopefun.jpg
    Mechanical Stage

    Knobs that move the platform stage from side to side/ forward and backward
  9. 18.
    http://www.personal.psu.edu/trp2/microscopeparts.jpeg
    Conderser lens

    lens system below the stage that concentrates the light reaching the specimen.

    Adjustement knob

    For raising and lowering the condenser is on side below stage
  10. 17.
    http://www.personal.psu.edu/trp2/microscopeparts.jpeg
    Iris diaphragm

    Lever on condenser that regulates amount of light entering specimen
  11. Course adjustment knob
    Large knob on both sides toward base used with 4x and 10x for initial focusing
  12. Fine adjustment knob
    • Small inner knob on both sides next to course adjustment knob
    • used for fine focus with 40x and 100x objective
  13. Euglena
    Cell Type/Organization/kingdom/Domain
    • Eukaryotic
    • Flagellated
    • Protista
    • Eukariote
  14. Anabaena
    Cell Type/Organization/kingdom/Domain
    • Prokaryotic
    • Filamentous
    • Eubacteria
    • Bacteria
  15. Spirogyra
    Cell Type/Organization/kingdom/Domain
    • Eukaryotic
    • Filamented
    • Protista
    • Eukaryote
  16. Paramecium
    Cell Type/Organization/kingdom/Domain
    • Eukaryotic
    • unicellular
    • Protista
    • Eukaryote
  17. Amoeba
    • Eukaryotic
    • unicelluar
    • Protista
    • Eukaryote
  18. Volvox
    • Eukaryotic
    • Plantae
    • Eukaryote
  19. Planaria
    • Eukaryotic
    • Multi-celluar
    • Fungi
    • Eukaryote
  20. Turbatrix
    • Eukaryotic
    • Filamented
    • Animalia
    • Eukaryote
  21. Aspergillus
    • Eukaryotic
    • Filamentous
    • Fungi
    • Eukaryote
  22. Rhyzopus
    • Eukaryotic
    • Fungi
    • Eukaryote
  23. Saccharomyce
    • Eukaryotic
    • Fungi
    • Eukaryote

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