Lab Quiz 3 Study Guide

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Lab Quiz 3 Study Guide
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2013-10-16 12:10:42
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Lab Quiz
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  1. TSI is useful in identifying the _____________ family of organisms.
    Enterobacteriaceae
  2. 3 genera of normal flora
    Erwinia, Proteus, Enterobacter
  3. Two species of organisms who are present only in the case of disease ______ and _____.
    Salmonella, Shigella
  4. Slant and butt both appear red:
    The reaction did not occur.
  5. Butt appears yellow while the slant appears red:
    The reaction occured, R/Y.
  6. The agar is crack and the butt and and slant are both yellow:
    The reaction occurred, Y/Y.
  7. Butt appears black while slant is yellow:
    The reaction did not occur.
  8. Aerotolerant anaerobe
    have SOD but only perform fermentations
  9. Aerobic
    uses oxygen as its final electron acceptor, do not ferment
  10. Obligate anaerobe
    have no SOD and catalase
  11. Facultative anaerobe
    can both ferment and use oxidative phosphorylation
  12. Microaerophile
    can only tolerate low levels of oxygen
  13. growth in a thioglycollate tube only occurs at the bottom of the tube:
    obligate anaerobes
  14. no growth occurs on a plate in the GasPak jar:
    obligate anaerobes, facultative anaerobes, and aerotolerant anaerobes
  15. growth occurs in a thioglycollate tube only at a few millimeters below the surface of the media:
    microaerophiles
  16. SOD (Superoxide dismutase)'s role in the cell and its final product(s)
    to convert superoxide free radicals (O2-) to O2 and H2O2
  17. Catalase's role in the cell and its final product(s):
    to neutralize peroxide, 2H2O and O2
  18. The final electron acceptor in aerobic respiration is ______________ while in anaerobic respiration the final electron acceptor is __________________________.
    O2 and inorganic compound
  19. Selective media are used to
    used to isolate specific groups of bacteria
  20. Two examples of selective media are
    Phenylethyl alcohol agar which selects for isolation of gram (+) bacteria and Crystal violet agar which selects for gram (-) bacteria
  21. Differential media are used to
    used to distinguish between biochemically & morphologically similar groups
  22. Two examples of differential media are
    Mannitol salt agar which differentiates based on manniotol fermentation and MacConkey agar which differentiates based on abaility to ferment lactose
  23. Tolerate 7.5% NaCl
    Staphylococcus
  24. That grow on MacConkey media but do not ferment lactose
    Salmonella and Shigella sp
  25. Are often distinguished by their different hemolytic properties
    Streptococcus
  26. No growth on mannitol salt agar (MSA) plate:
    negative = no color change in media.
  27. Growth on a mannitol salt (MSA) agar plate; media turns yellow
    positive = media turns yellow
  28. Why must the E.coli be heat shocked to introduce the plasmid DNA?
    The E.coli must be head shocked to introduce the plasmid DNA in order to make the E.coli cells competent to accept DNA
  29. What is the role of ampicillin in the culture media?
    to select colonies containing the plasmid
  30. What is the purpose of the plate containing only LB agar without ampicillin or arabinose?
    to show E.coli survived the procedure
  31. What is the purpose of the arabinose in the LB arabinose plates?
    to make transformed bacteria to glow green
  32. What is the purpose of the plate containing ampicillin, but plated with the untransformed pGLO minus bacteria?
    to leave only the transformed bacteria to survive
  33. Morphological methods (simple stains, colony morphology, etc)
    • Strength: easy to perform; quick results
    • Weakness: easy to misinterpret, can result in false positive and negative results occur
  34. Differential staining (Gram, endospore, acid fast)
    • Strength: divides organisms into definitive groups; can identify genus
    • Weakness: can be difficult to perform
  35. Biochemical tests (Enzyme/metabolic assays)
    • Strength: can identify genus and species
    • Weakness: requires multiple tests; time for growth
  36. Briefly explain why the absence of endospores and/or the absence of a capsule are NOT definitive evidence for the identification of a bacteria.
    The absence of endospores and/or the absence of a capsule are not definitive evidence for the identification of a bacteria because false positives and negatives occur and positive pigment formation may be used as a final confirmation test if NO other test is available.
  37. Staphylococcus aureus
    enteritis
  38. Staphylococcus epidermidis
    endocarditis
  39. Staphylococcus saprophyticus
    urinary tract infections
  40. What does a rose halo around the bacterial growth on a DNA agar plate tell you about the organism?
    positive result = (+) DNase/ (+) DNA hydrolysis
  41. Which bacterium is identified by being DNase and coagulate positive?
    S.aureus

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