microscopy

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Author:
melissag94
ID:
239920
Filename:
microscopy
Updated:
2013-11-06 15:21:09
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BIO100 exam
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lab questions
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  1. all microscopes have objective lenses, t of f?
    true
  2. which type of microscope creates an image by capturing electrons ejected from the specimen?
    SEM
  3. which type of microscope can be used to observe living cells and tissues
    LM
  4. which type of microscope has the greatest resolving power?
    electron microscope
  5. how small is the smallest object that can be seen with a light microscope?
    .2 um
  6. how small is the smallest object that can be observed using a transmission electron microscope
    .2nm
  7. why do electron microscopes have the ability to resolve such small objects?
    electrons have a much shorter wavelength than the photons of visible light
  8. which type of microscope would be used to observe structures on the surface of an individual cell
    SEM
  9. which type of cell does not have a cell wall?
    animal
  10. which microscope allows the use of color to enhance contrast?
    light
  11. which type of microscope reveals the most fine detail of internal cellular structure
    transmission electron
  12. which organelles are visible with the light microscope?
    nucleus, nucleolus, vacuole
  13. to study the fine structure of a mitochondrion, which type of microscope is least appropriate?
    light
  14. which type of microscope provides the greatest depth of field?
    scanning electron
  15. which type of microscope is used for observing small surface detail
    scanning electron
  16. as a tissue, blood is composed of only one type of cell, t or f?
    false
  17. which type of cell does not have a cell wall?
    animal
  18. chloroplasts are a feature of
    plant cells
  19. mitochondria are about the same size as bacterial cells, t or f?
    true
  20. are plant and animal cells generally the same size?
    no, plant cells are generally larger
  21. plant and animal cells are generally ____ bacterial cells
    larger than
  22. are all nuclei the same size?
    no
  23. are plant nuclei always larger than animal nuclei
    no
  24. which cell type has the largest nucleus
    neuron
  25. Which type of microscope can be used to directly observe cells and tissues?
    light
  26. which type of microscope can be used to observe very small subcellular structures?
    transmission electron
  27. which type of microscope uses chemical stains to add color and increase contrast?
    light
  28. which type of microscope can be used to observe very small surface details?
    scanning electron
  29. in general, light microscopes can magnify an object up to approximately how many times?
    1000
  30. in general, bacterial cells are smaller than the cells of eukaryotic plant and animals?
    true
  31. in electron microscopy, the specimens are stained with heavy metals such as lead or uranium, t or f?
    true
  32. all microscopes are designed to magnify objects too small to be seen with the naked eye, t or f?
    true
  33. the resolving power of a microscope is ___ related to the wavelength of radiation being used to illuminate the specimen
    inversely
  34. when using a light microscope, you actually look through the
    ocular
  35. all microscopes have objective lenses, t or f?
    true
  36. staining is used to increase
    contrast
  37. in general, objects smaller than a bacterial cell cannot be seen using a light microscope
    true
  38. if a cell has a diameter of 100 um, what is its diameter when measure using a microscope that magnifies 100X?
    100 um
  39. which type of microscope reveals the most subcellular detail?
    TEM
  40. nuclei are too small to be seen with a light microscope, t or f?
    false
  41. based on your observations, which type of cell has the largest nucleus?
    neuron
  42. bacterial cells possess a cell wall, t or f?
    true
  43. frequently, the largest organelle in a cell is the /
    nucleus
  44. as small cellular extensions, cilia would be best observing using a?
    scanning electron microscope
  45. which type of microscope uses a detector to collect electrons ejected from the specimen?
    SEM
  46. nuclei can be observed using light, transmission electron, or scanning electron microscopes, t or f?
    true
  47. to observe a living amoeba, one would use a?
    light microscope
  48. do bacterial cells have a nucleus?
    no
  49. blood is a tissue composed of only one type of cell, t or f?
    false

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