Mcim L10 Gene Expression

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Scottygo
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240391
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Mcim L10 Gene Expression
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2013-10-13 17:50:12
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Mcim L10 Gene Expression
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Mcim L10 Gene Expression
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  1. REPLICATION TRANSCRIPTION AND TRANSLATION
    Eukaryotes
    • -EUKAROTES HAVE REPLICATION AND TRANSCRIPTION IN THE NUCLEUS
    • – TRANSLATIONIS IN THE CYTOPLASM
  2. REPLICATION TRANSCRIPTION AND TRANSLATION
    Prokaryotes
    4 differences
    • -PROKARYOTES HAVE COUPLED TRANSCRIPTION & TRANSLATION
    • -TRANSCRIPTION & TRANSLATION OF A GENE AT THE SAME TIME AND IN THE SAME COMPARTMENT.
    • -SIGMA FACTORS CONTROL INITIATION OF TRANSCRIPTIONP PROMOTER SEQUENCES OF PROKARYOTES ARE DIFFERENT FROM THOSE IN EUKARYOTES.
    • -70 S RIBOSOMES OF PROKARYOTES ARE DIFFERENT. 
    • -SD SITES (RIBOSOMAL BINDING SITES) CONTROL INITIATION OF TRANSLATION IN PROKARYOTES, BUT NOT IN EUKARYOTES.
  3. PROMOTERS
    A PROMOTER IS A SEQUENCE OF BASES IN THE DNA WHICH ACTS AS A SIGNAL TO START TRANSCRIPTION OF RNA.
  4. RNA POLYMERASE
    AN ENZYME WHICH SYNTHESISES mRNA FROM A DNA TEMPLATE
  5.  FACTOR
    • -ENABLES RNP TO BIND TO SPECIFIC PROMOTERS AND TO INITIATE TRANSCRIPTION
    • -Not required for elongation
    • -DIFFERENT  FACTORS RECOGNISE DIFFERENT PROMOTER SEQUENCES
  6. 4 steps to transcription
    • -RNP binds  factor
    • -RNP binds DNA (closed complex)
    • -RNP maneuvers between DNA strands (open complex)
    • -Elongation
  7. At what point in the initiation of transcription is it no longer reversible
    open complex
  8. CONSENSUS SEQUENCES
    def
    example
    A sequence in DNA that is constant when compared in multiple  strands of DNA

    -Promoters found at -35 and -10 positions
  9. What is the -35 sequence?
    TTG_ _ _
  10. What is the -10 sequence?
    TA_ _ _T
  11. WEAK PROMOTERS
    • -DO NOT MATCH THE CONSENSUS SEQUENCE AS WELL AS STRONG PROMOTERS
    • -REGULATED BY ACTIVATOR PROTEINS
  12. STRONG PROMOTERS
    • -Match consensus sequence better than weak promoters
    • -Turned off by repressors
  13. PROMOTER MUTATIONS .
    CHANGE THE DNA SEQUENCE IN THE CONSENSUS REGIONS
  14. PROKARYOTIC RIBOSOMES
    ___s size
    P TWO SUBUNITS(___S AND ___S) 
    ___PROTEINS  
    _RNA’S 
    __S RNA BINDS mRNA FOR TRANSLATION
    TARGET FOR ___
    • 70
    • 50 30
    • 51
    • 3
    • 16
    • ANTIBIOTICS
  15. Initiation:
    Shine Dalgarno Sequence
    Site on mRNA just before start codon that 16S rRNA (in 30S subunit) binds to
  16. Initiation:
    Action of Ribosome subunits
    50 S SUBUNIT BINDS TO 30S SUBUNIT FROM OTHER SIDE OF mRNA TO FORM 70S RIBOSOME
  17. Why does 30S + 50S=70S?
    50S subunit has groove that 30S fits into
  18. INITIATION
    What binds to AUG start codon?
    N-FORMYLMETHIONINE-tRNA
  19. TRANSLATION:
    What Happens after start codon Is bonded?
    • -SECOND AMINO ACYL-tRNA BINDS TO NEXT CODON AND RIBOSOMEFORMS PEPTIDE BOND
    • -Elongation continues
    • -At stop codon translation ends and ribosomal subunits are released

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