Micro Chapter 9

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  1. sterilization
    removal or destruction of all harmful organisms
  2. disinfection
    removing or destroying most organisms on inanimate objects
  3. antisepsis
    removing or destroying on the surfaces of living organisms
  4. degerming
    to physically remove organisms from the skin
  5. pasteurization
    heat to reduce the number of organisms in foods or beverages
  6. decimal reduction time
    how long it takes to kill 90 percent of the population
  7. Boiling
    good for control of microbes

    not good for endospores, protozoan cysts, some viruses
  8. Autoclaving
    • True sterilization
    • works of endospores
  9. Dry heat
    used for substances or articles that would be damaged by boiling or steam.
  10. Batch pasteurization
    • 30 minutes at a time
    • 63 degrees C
  11. Flash pasteurization
    • 72 degrees celcius
    • 15 seconds
  12. Ultrahigh-temperature sterilization (pasteurization)
    • 140 degrees celciusĀ 
    • 1-3 seconds
  13. Refrigeration
    inhibit by slowing metabolism and reducing growth and reproduction
  14. slow freezing
    inhibit most organisms because of the damage caused by ice crystals formed inside the cells
  15. desication (drying)
    inhibits growth because metabolism requires water. effective for most pathogens but not molds
  16. lyophilization
    frozen to remove water crystals. Allows for preservation for many years
  17. nonionizing radiation
    UV light. good for disinfecting air, transparent fluids and surfaces
  18. Ionizing radiation
    electron beams, gamma rays, x rays. Used on fresh foods to kill parasites and prevent spoilage
  19. Osmotic pressure
    jellies and jerky
  20. Common resistant microbes
    • Escherichia coli
    • Mycobacterium tuberculosis
    • Pseudomonas aeruginosa
    • Staphylococcus aureus
  21. Phenol Coefficient
    Compare a disinfectant to phenol coefficient effectiveness. If the phenol coefficient is greater than 1 than the agent tested is more effective than phenol. If the coefficient is less than one than it is less efficient
  22. Use-Dilution Test
    tests agents against specific organisms. Known amounts of organisms are exposed to differing dilutions of the agent begin tested for 10 MINUTES. Then placed in sterile broth and incubated. Tube with lowest turbidity wins
  23. Kelsey-Sykes Capacity test
    how long it takes a particular concentration of the agent being tested to inhibit the growth of the organism. Suspension added to agent and at time intervals a sample is removed and added to broth media which also contains a disinfectant deactivator. Lack of turbidty = inhibition
  24. In-Use test
    uses samples taken before treatement and after treatment to try and get a more realistic idea of how well the agent works
Card Set
Micro Chapter 9
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