Microscope Quiz

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Author:
adriana321
ID:
242006
Filename:
Microscope Quiz
Updated:
2013-10-22 10:07:27
Tags:
Microscope Biology
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Description:
Microscope
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  1. Purpose of Microscope
    to see inside of organisms and cells;what is invisible to the naked eye
  2. Compound Microscope
    • has two lenses at opposite ends of tube
    • for smaller things
  3. Ocular Lens
    • top
    • 10x life size; what you look through
  4. Body Tube
    • under the ocular lens
    • connects arm to the ocular lens
  5. Revolving Nosepiece
    • between objectives and body tube
    • moves the objectives
  6. Objective Lens
    • connected to the revolving nosepiece
    • lens nearest object/specimen
  7. Stage
    • under objective lens
    • where specimen is placed
  8. Stage Clips
    • on stage
    • holds the microscope slide, keeps specimen in place
  9. Diaphragm
    • below stage
    • allows the light to pass through the stage
  10. Light Source
    • on button of microscope
    • source of light
  11. Light Switch
    • back of microscope
    • turns on light
  12. Coarse Adjustment Knob
    • side of arm
    • bring specimen to view
    • used under low power, if used under high power can crack the slide
  13. Fine Adjustment Knob
    • side of arm
    • focus the view
    • used to focus under high power
  14. Arm
    • back
    • holds longest stage
  15. Base
    • bottom
    • supports microscope
  16. Dissecting Microscope
    • binocular= 2 ocular lenses; 3D
    • for larger objects
  17. Magnification
    measure of how big an object looks to your eye compared to life size
  18. Total Magnification
    multiply the ocular lens(10x) by power of the objective lens
  19. Resolution/Resolving Power
    • measure how clearly you can see details in the microscope
    • is usually given as distance between two objects that can barely be resolved
  20. Field of View
    gets smaller as magnification increases
  21. Point of Interest
    • what you are looking at
    • if it is not exactly centered in low magnification, will not be visible at higher magnifications
    • if not visible, return to low power, center, switch to high power
  22. Depth of Field
    • decreases as field of view becomes smaller
    • thickness of specimen is larger under low power, easier to focus using coarse adjustment
    • thin layer is in focus under higher magnification, use fine adjustment
  23. Microbes
    • organisms too small to be seen in any detail with the naked eye
    • 1 micrometer= 1/1000 of a millimeter 
    • human eye can see objects 100 micrometers or larger
  24. Measuring Objects
    • a= field size at 100x in mm
    • b= low magnification(100x)
    • c= size of the field you are trying to determine
    • d= magnification of field you are trying to determine
    • Ex) a=3mm b=100 c=? d=400x

    compare size of object to size of field, if object is 1/4 of diameter of field 

    0.25* 750= 187.5 micrometers
  25. Transmission Electron Microscope
    • can actually cut through 
    • can see "inside"
  26. Scanning Electron Microscope
    • scans surface
    • gives 3D appearance 

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