Microbio Pre-lab 5 Quiz
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What is the component in acid-fast organisms that makes the stain possible ?
Is Mycolic acid a waxy substance ?
What is the difference between the Ziehl-Neelsen method (ZN) and the Kinyoun method (K) ?
The ZN method used heat in the staining process and the K method is cold
Why is the sheep serum used instead of water ?
The acid-fast organisms is slippery , so it adheres to the sheep serum.
When acid-fast organisms repels most stains what are they seen as ?
Weakly gram positive
Why is carbolfuchsin used as the primary stain ?
It is a phenolic compound that penetrates the waxy cell wall
Why is heat applied in the Ziehl-Neelsen method ?
It allows melts the waxy layer and allows the stain to move into the cell.
What is the decolorizer for ?
It is for non acid-fast organisms. The acid-fast organisms can resist the decolorization
What color are Acid fast organisms ?
What color will non-acid fast organisms be ?
The color of the secondary stain used .
How does the Kinyoun method work ?
It uses a stronger carbolfuchsin stain that penetrates the waxy cell , but it is not as effective as the heat.
What genus is the acid-fast used for ?
Actinomycete organisms and
Oocysts of coccidian parasites
What morphology is Acid Fast typically used for ?
Acid -Fast Bacilli (AFB) - has a 90 % predictive value.
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